Protocol for tumor detection in mice using β-glucuronidase targeted breath analysis.

Guochao Wu, Tianran Si, Han Jin

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Guochao WuInstitute of Nano Biomedicine and Engineering & National Key Laboratory of Advanced Micro and Nano Manufacture Technology, School of Automation and Intelligent Sensing, Shanghai Jiao Tong University, Shanghai 200240, P.R. China.

Tianran SiInstitute of Nano Biomedicine and Engineering & National Key Laboratory of Advanced Micro and Nano Manufacture Technology, School of Automation and Intelligent Sensing, Shanghai Jiao Tong University, Shanghai 200240, P.R. China.

Han JinInstitute of Nano Biomedicine and Engineering & National Key Laboratory of Advanced Micro and Nano Manufacture Technology, School of Automation and Intelligent Sensing, Shanghai Jiao Tong University, Shanghai 200240, P.R. China; National Engineering Research Center for Nanotechnology, Shanghai 200241, P.R. China. Electronic address: jinhan10@sjtu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Early tumor detection is critical for improving cure rates. Here, we present a protocol for tumor detection in mice using a targeted breath-analysis strategy in which phenyl-β-D-glucuronide specifically targets β-glucuronidase, which is highly expressed in the tumor microenvironment. We describe steps for drug screening, enzymatic reaction, drug stability and toxicity evaluation, and construction of the mouse exhaled-breath extraction device. We then detail procedures for animal-model breath analysis, enabling highly sensitive early tumor detection. For complete details on the use and execution of this protocol, please refer to Xue et al.

Indexed as

CancerChemistryHealth Sciences

Identifiers

PMID42560813

DOI10.1016/j.xpro.2026.104764

PMCPMC13471240

What OpenQuestion holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.