Protocol to investigate the role of NETosis in health and disease via a high-throughput neutrophil-like model using quantitative imaging.

Gavin Li, Sara Versales, Brett Guadagnino, Kavya Krishnan, Michele Matsuoka, Hyesun Jang, Marcelo Freire

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Gavin LiDepartment of Human Biology and Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.

Sara VersalesDepartment of Human Biology and Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.

Brett GuadagninoDepartment of Human Biology and Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.

Kavya KrishnanDepartment of Human Biology and Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.

Michele MatsuokaDepartment of Human Biology and Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.

Hyesun JangDepartment of Human Biology and Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA.

Marcelo FreireDepartment of Human Biology and Genomic Medicine, J. Craig Venter Institute, La Jolla, CA 92037, USA; Department of Infectious Disease, J. Craig Venter Institute, Rockville, MD 20850, USA; Division of Infectious Diseases and Global Public Health, University California, San Diego, La Jolla, CA, USA. Electronic address: mfreire@jcvi.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neutrophil extracellular traps (NETs) are a crucial host-defense mechanism employed by neutrophils in response to various aggregates and contribute to pathology in diverse diseases, from chronic inflammation to sepsis. Here, we present a protocol to investigate the role of NETosis, the release of NETs, in health and disease via a high-throughput neutrophil-like model using quantitative imaging. We describe steps for preparing stimulated, differentiated Human Leukemia-60 (HL-60) cells; immunostaining; high-throughput microscopy; and NETosis quantification using CellProfiler.

Indexed as

Health SciencesHigh Throughput ScreeningImmunologyMicroscopy

Identifiers

PMID42560812

DOI10.1016/j.xpro.2026.104726

PMCPMC13471202

What OpenQuestion holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.