ArticleSTAR protocols2026
Protocol to determine oligomerization states of proteins in living cells using photon counting histogram analysis.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 authors.
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Abstract
Determining protein oligomerization states in living cells is essential for understanding cell signaling and disease pathology, but it remains technically challenging. Here, we present a protocol for quantifying the oligomeric states of HaloTag-labeled proteins in living mammalian cells using fluorescence fluctuation spectroscopy (FFS) and photon counting histogram (PCH) analysis. Using the photoactivatable protein cryptochrome 2 as a representative model, we describe steps for cell culture, chemical transfection, cell staining, and FFS data acquisition. We then detail procedures for PCH analysis. For complete information on the generation and use of this protocol, please refer to Camp et al.
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