Evidence map›Paper›PMID 42560808›Full record

ArticleSTAR protocols2026

Protocol to improve gradient-index lens implantation in the mouse medial prefrontal cortex using NeuralGlider-assisted insertion.

Sishir Gautam, Olga M Ocón-Grove, Jenna K Greaser, Navin Adhikari, Rashmi Thapa, Yun Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sishir GautamDepartment of Zoology and Physiology, University of Wyoming, 1000 E. University Avenue, Laramie, WY 82071, USA.
Olga M Ocón-GroveActuated Medical, Inc., 320 Rolling Ridge Drive, Bellefonte, PA 16823, USA.
Jenna K GreaserActuated Medical, Inc., 320 Rolling Ridge Drive, Bellefonte, PA 16823, USA.
Navin AdhikariDepartment of Zoology and Physiology, University of Wyoming, 1000 E. University Avenue, Laramie, WY 82071, USA.
Rashmi ThapaDepartment of Zoology and Physiology, University of Wyoming, 1000 E. University Avenue, Laramie, WY 82071, USA.
Yun LiDepartment of Zoology and Physiology, University of Wyoming, 1000 E. University Avenue, Laramie, WY 82071, USA. Electronic address: yli30@uwyo.edu.

Funding

Loss of TDP-43 disrupts the prefrontal neural activity and circuitry: relevance for TDP-43 linked ADRDR01NS129878 · NINDS · UNIVERSITY OF WYOMING · PI YUN LI, PHILIP C WONG · 2025 to 2026
$1.5M
Integrating Experimental and Computational Models to Study the Prefrontal Microcircuit Changes During Aging and Alzheimer's DiseaseR33AG087909 · NIA · UNIVERSITY OF WYOMING · PI RONG CHEN, YUN LI · 2026 to 2026
$717k
Diversity Supplement for Development of an Oscillated Insertion tool to Eliminate Surgically Induced Neurodegeneration for Optical Neuroimaging of Cognitive Aging and DementiaR43AG084801 · NIA · ACTUATED MEDICAL, INC. · PI GHERES, KYLE WILLIAM · 2023 to 2024
$456k
Integrating Experimental and Computational Models to Study the Prefrontal Microcircuit Changes During Aging and Alzheimer's DiseaseR21AG087909 · NIA · UNIVERSITY OF WYOMING · PI CHEN, RONG, LI, YUN · 2024 to 2025
$386k
NIA NIH HHS R21 AG087909NIA NIH HHS R33 AG087909NIA NIH HHS R43 AG084801NINDS NIH HHS R01 NS129878
6 · The paper itself

Abstract

Gradient-index (GRIN) lens implantation enables deep brain imaging in animal models. Tissue-aspiration based GRIN lens implantation procedures often suffer from low success rates. Here, we present an improved protocol for NeuralGlider-assisted GRIN lens implantation in the mouse medial prefrontal cortex. We describe steps from viral injection to in vivo calcium imaging, with primary focus on GRIN lens implantation. This NeuralGlider-assisted insertion protocol minimized tissue damage through a controlled insertion speed, gentle lens rotation, axial micro-oscillation, and vacuum-based lens stabilization.

Indexed as

Biotechnology and bioengineeringNeuroscienceTissue Engineering

Identifiers

PMID42560808
PMCPMC13476365

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.