Evidence map›Paper›PMID 42557105›Full record

ArticleeNeuro2026

A Streamlined Workflow for Purkinje Cell Labeling and High-Resolution Analyses of Dendrites and Spines in Mice.

Xinzhu Tan, Simeng Niu, Melissa Bai, Platon Megagiannis, Rahul Suresh, Elias Pilianidis, Yosuke Niibori, David R Hampson, Thomas M Durcan, Yang Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eNeuro, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xinzhu TanDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3A 2B4, Canada.ORCID https://orcid.org/0000-0002-1488-8421
Simeng NiuDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3A 2B4, Canada.
Melissa BaiDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3A 2B4, Canada.
Platon MegagiannisDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3A 2B4, Canada.
Rahul SureshDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3A 2B4, Canada.
Elias PilianidisDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3A 2B4, Canada.
Yosuke NiiboriRe:Pair Genomics Inc., Vaughan, Ontario L6A 3M2, Canada.
David R HampsonLeslie Dan Faculty of Pharmacy, Department of Pharmaceutical Sciences, University of Toronto, Toronto, Ontario M5S 3M2, Canada.
Thomas M DurcanDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3A 2B4, Canada.ORCID https://orcid.org/0000-0003-3942-1956
Yang ZhouDepartment of Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, Faculty of Medicine and Health Sciences, McGill University, Montreal, Quebec H3A 2B4, Canada yang.zhou7@mcgill.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cerebellar Purkinje cells (PCs) exhibit a unique and highly complex dendritic architecture, which plays a crucial role in integrating synaptic inputs and shaping their physiological properties. However, fine morphological investigation of PCs at the single-cell level is challenging due to the densely packed soma and extensive dendritic arborization. Here, we present stepwise procedures for the sparse labeling of PCs via a viral-mediated approach, enabling detailed two-dimensional dendritic Sholl analysis and three-dimensional spine quantification. We demonstrated high-resolution labeling of PCs through the administration of an adeno-associated virus (AAV) carrying a conditional Cre recombinase-dependent cassette (AAV,

Indexed as

DendritesDendritic SpinesPurkinje CellsStaining and LabelingAnimalsDependovirusFemaleGreen Fluorescent ProteinsImaging, Three-DimensionalMaleMiceMice, Inbred C57BLMice, TransgenicGreen Fluorescent Proteins3D reconstructioncell labelingcerebellumCre-dependent AAVdendritic spinedendritic treeDravet syndromemicePurkinje cellSholl analysis

Identifiers

PMID42557105
PMCPMC13505807

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.