Evidence map›Paper›PMID 42557056›Full record

ArticleRapid communications in mass spectrometry : RCM2026

Systematic Optimization of an SLE-DAPTAD LC-MS/MS Workflow for Simultaneous Quantification of Low-Abundance Vitamin D

Marzieh Amirmostofian, Michael D Wiese, Paul H Anderson, Peter Hoffmann

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In one paragraph

Article in Rapid communications in mass spectrometry : RCM, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Marzieh AmirmostofianMass Spectrometry and Proteomics, College of Health, School of Pharmacy and Biomedical Science, Adelaide University, Adelaide, South Australia, Australia.ORCID https://orcid.org/0000-0002-2705-4602
Michael D WieseCollege of Health, School of Pharmacy and Biomedical Science, Adelaide University, Adelaide, South Australia, Australia.
Paul H AndersonCollege of Health, School of Pharmacy and Biomedical Science, Adelaide University, Adelaide, South Australia, Australia.
Peter HoffmannMass Spectrometry and Proteomics, College of Health, School of Pharmacy and Biomedical Science, Adelaide University, Adelaide, South Australia, Australia.ORCID https://orcid.org/0000-0001-8493-2208

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

rationaleLiquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of vitamin D

methodsFive sample-preparation approaches-simple protein precipitation, liquid-liquid extraction, salting-out assisted liquid-liquid extraction, solid-phase extraction, and supported liquid extraction-were evaluated under comparable conditions. Two derivatization reagents, 4-(4'-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD) and 2-fluoro-1-methylpyridinium-p-toluenesulfonate (FMP-TS), were compared. The selected workflow was validated according to bioanalytical method validation criteria.

resultsSupported liquid extraction using ethyl acetate/methyl tert-butyl ether (1:1, v/v) provided the best overall balance of signal intensity, reproducibility, simplicity, and suitability for high-throughput processing. DAPTAD derivatization produced a substantially higher MS response than FMP-TS and was selected for the final method. The optimized SLE-DAPTAD LC-MS/MS workflow showed good linearity (r

conclusionsThe optimized SLE-DAPTAD LC-MS/MS workflow provides a simple, sensitive, and automation-compatible approach for simultaneous quantification of four vitamin D

Indexed as

CholecalciferolLiquid Chromatography-Mass SpectrometryTandem Mass SpectrometryAniline CompoundsChromatography, LiquidHumansLimit of DetectionLiquid-Liquid ExtractionReproducibility of ResultsSolid Phase ExtractionTriazoles4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dioneAniline CompoundsCholecalciferolTriazolesDAPTAD derivatizationLC–MS/MSmethod optimizationsample preparationsupported liquid extractionvitamin D3 metabolites

Identifiers

PMID42557056
PMCPMC13441545

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.