Evidence map›Paper›PMID 42556348›Full record

ArticleMolecular cell2026

α-Satellite RNA maintains centromeric cohesion through R-loops.

Lu Yang, Qian Zhang, Zhen Teng, Erin Moran, Zhen Lin, P Todd Stukenberg, Hong Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Lu YangDepartment of Biochemistry & Molecular Biology, Tulane University School of Medicine, 1430 Tulane Ave., New Orleans, LA 70112, USA.
Qian ZhangDepartment of Biochemistry & Molecular Biology, Tulane University School of Medicine, 1430 Tulane Ave., New Orleans, LA 70112, USA.
Zhen TengDepartment of Biochemistry & Molecular Biology, Tulane University School of Medicine, 1430 Tulane Ave., New Orleans, LA 70112, USA.
Erin MoranDepartment of Biochemistry and Molecular Biology, Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22903, USA.
Zhen LinTulane Cancer Center, Tulane University School of Medicine, New Orleans, LA 70112, USA; Department of Pathology & Laboratory Medicine, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans, LA 70112, USA.
P Todd StukenbergDepartment of Biochemistry and Molecular Biology, Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA 22903, USA.
Hong LiuDepartment of Biochemistry & Molecular Biology, Tulane University School of Medicine, 1430 Tulane Ave., New Orleans, LA 70112, USA; Tulane Cancer Center, Tulane University School of Medicine, New Orleans, LA 70112, USA; Tulane Aging Center, Tulane University School of Medicine, New Orleans, LA 70112, USA. Electronic address: hliu22@tulane.edu.

Funding

Cancer Research Training Program: From Molecular Mechanisms to Therapeutic StrategiesT32CA009109 · NCI · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI Andrew Carl Dudley, Melanie R Rutkowski · 1985 to 2026
$13.9M
Mechanisms of mitotic regulationR35GM148181 · NIGMS · UNIVERSITY OF VIRGINIA · PI P. TODD STUKENBERG · 2023 to 2026
$2.9M
Vitamin D in viral associated lung cancersR01CA261258 · NCI · TULANE UNIVERSITY OF LOUISIANA · PI LIN, ZHEN · 2021 to 2025
$1.9M
Timing Control of Centromeric Cohesion through Centromere-localized Sgo1R01GM124018 · NIGMS · TULANE UNIVERSITY OF LOUISIANA · PI LIU, HONG · 2018 to 2022
$1.6M
Functions and Regulation of Centromeric TranscriptionR01GM141123 · NIGMS · TULANE UNIVERSITY OF LOUISIANA · PI LIU, HONG · 2021 to 2024
$1.2M
Alpha-satellite RNAs in Epstein-Barr virus lytic replicationR01AI195576 · NIAID · TULANE UNIVERSITY OF LOUISIANA · PI Zhen Lin, Hong Liu · 2026 to 2026
$572k
NCI NIH HHS R01 CA261258NCI NIH HHS T32 CA009109NIAID NIH HHS R01 AI195576NIGMS NIH HHS R01 GM124018NIGMS NIH HHS R01 GM141123NIGMS NIH HHS R35 GM148181
6 · The paper itself

Abstract

Tandemly repeated satellite DNAs provide the building blocks for centromeres, but whether and how their transcripts regulate chromosome segregation remain poorly defined. Here, we show that human α-satellite RNAs (α-SatRNAs) are required for maintaining centromeric cohesion during mitosis. α-SatRNAs localize to centromeres and physically associate with α-satellite chromatin to form R-loops both in cis and in trans. Disruption of these R-loops by RNase H1 overexpression causes centromeric cohesion defects, phenocopying α-SatRNA knockdown, which can be rescued by the expression of an α-satellite consensus sequence, supporting a major role for α-SatRNA-formed R-loops in centromeric cohesion. Furthermore, ectopic targeting of the cohesion protector Shugoshin (Sgo)1 to centromeric α-SatDNAs largely rescues centromeric cohesion defects caused by α-SatRNA knockdown or RNase H1 overexpression. Our findings reveal a critical pathway comprising α-SatRNAs, R-loops, and Sgo1 in regulating centromeric cohesion, thus providing an important mechanism to explain how satellite transcripts regulate chromosomal stability.

Indexed as

CentromereR-Loop StructuresRNA, SatelliteCell Cycle ProteinsChromosomal Proteins, Non-HistoneChromosome SegregationDNA, SatelliteHeLa CellsHumansMitosisRibonuclease HCell Cycle ProteinsChromosomal Proteins, Non-HistoneDNA, SatelliteRibonuclease Hribonuclease HIRNA, SatelliteSGO1 protein, humancentromerecentromeric cohesionchromosome segregationR-loopsα-satellite RNA

Identifiers

PMID42556348
PMCPMC13532348

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.