ArticleStem cells translational medicine2026
Differential miRNA profiles in fluoride-exposed rBMSCs-EVs and the influence of miR-320-3p transfer on osteogenic differentiation.
Article in Stem cells translational medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Long-term excessive fluoride ingestion may lead to skeletal fluorosis, a disease characterized by unclear pathogenesis and lack of effective cure. Bone marrow mesenchymal stem cell-derived extracellular vesicles (BMSCs-EVs) exert a crucial regulatory effect on bone remodeling and have broad clinical application prospects. Whether BMSCs-EVs can participate in the occurrence and development of skeletal fluorosis by transferring miRNAs is a scientific question worth exploring. In this study, miRNA sequencing was used to screen for differentially expressed miRNAs in fluoride-exposed rBMSCs-EVs (F-EVs). The top 15 differentially expressed miRNAs were subjected to target gene prediction, and GO and KEGG analyses as well as PPI network construction were performed. On this basis, miR-320-3p was selected for in-depth study. First, our data revealed that miR-320-3p was upregulated in fluoride-exposed osteoblasts and was involved in the enhancement of fluoride-induced osteogenic differentiation. Subsequently, a tracing experiment confirmed that rBMSCs-EVs and their miR-320-3p could be successfully taken up by fluoride-exposed osteoblasts. Then, elevated levels of miR-320-3p in F-EVs could further promote the enhancement of fluoride-induced osteogenic differentiation, while EVs with inhibited miR-320-3p exerted a significant inhibitory effect. Mechanism studies revealed that under fluoride exposure, rBMSCs-EVs could regulate Igf1r/ERK1/2/MAPK by transferring miR-320-3p, thereby affecting osteogenic differentiation. This study is the first to elucidate the pathogenesis of skeletal fluorosis from the rBMSCs-EVs miRNAs and provides new ideas for the clinical management of skeletal fluorosis.
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