Evidence map›Paper›PMID 42555688›Full record

ArticleThe Indian journal of medical research2026

Zika virus positivity in Aedes aegypti mosquitoes and its genomic landscape from Pune, Western India.

Sreelakshmi P R, Mehrunnisa Raje, Surendra Kumar, Tanwee Das, Irrusappan Hari, Mahendra Jagtap, Ashwini Ramdasi, Supriya Hundekar, Sarah Cherian, Atul M Walimbe and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Indian journal of medical research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Sreelakshmi P RDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.
Mehrunnisa RajeDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.
Surendra KumarDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.
Tanwee DasDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.
Irrusappan HariDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.
Mahendra JagtapDepartment of Vector Borne Diseases, National Centre for Vector Borne Disease Control, Maharashtra, India.
Ashwini RamdasiDepartment of Hepatitis, ICMR-National Institute of Virology, Pune, India.
Supriya HundekarDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.
Sarah CherianDepartment of Bioinformatics, ICMR-National Institute of Virology, Pune, India.
Atul M WalimbeDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.
Sachin DhaigudeDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.
Prafulla SonawaneDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.
Vinayak IngaleDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.
Gajanan N SapkalDepartment of Diagnostic Virology Group, ICMR-National Institute of Virology, Pune, India.
Kavita LoleDepartment of Entomology, ICMR-National Institute of Virology, Pune, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and objectives Zika virus cases were confirmed from Pune, Western India, during an outbreak in June 2024. We conducted an entomological investigation in the Pune municipal corporation area and aimed to characterise the Zika virus from Aedes aegypti mosquitoes at the genomic level. Methods An entomological survey was conducted covering 16 locations in the Pune Municipal Corporation, between June and August 2024. Adult mosquitoes were collected using aspirators, while larvae were collected with the standard dipping and pipetting method. Aedes vector density was assessed, and samples were pooled. Zika virus positivity was assessed using real-time RT-qPCR. Genomic characterisation of the strains was achieved by whole-genome sequencing using the Oxford Nanopore technology platform. Results A high density of Aedes mosquitoes was revealed in the Pune area. Zika virus positivity was observed in 34/128 (26.5%) of Aedes aegypti mosquito pools. Full genome sequences of two ZIKV isolates were recovered from adult mosquito pools. Their time-scaled phylogenetic analysis showed grouping within the Asian lineage, having a recent ancestral time of evolution around 2014, indicating the introduction of newer virus strains in the recent past in India. Mutations E-V473M and motif E-VNDT, indicative of faster viral replication and higher mosquito infectivity, were noted in only one of the two isolates. Interpretation and conclusions With a high positivity of the Zika virus in Aedes aegypti, this study highlights the ongoing risk prevailing in the Pune municipal corporation area. Distinct characteristics of the circulating strains of the virus, and their evolutionary links to different global strains, underlines the importance of continuous genomic surveillance in vectors and hosts.

Indexed as

AedesMosquito VectorsZika VirusZika Virus InfectionAnimalsDisease OutbreaksGenome, ViralHumansIndiaPhylogenyWhole Genome SequencingAedes aegyptiEntomological surveillanceOutbreakWhole genome sequencingZika virus characterisation

Identifiers

PMID42555688
PMCPMC13466802

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.