Protocol for conditional mapping of proximal interactomes of dimeric protein complexes using a BioID-based complementation approach.

Abishankari Rajkumar, Antoine Gaudreau-Lapierre, Curtis L F Anthony, Laura Trinkle-Mulcahy

Erratum issuedAbstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

Erratum issued
Protocol for conditional mapping of proximal interactomes of dimeric protein complexes using a BioID-based complementation approach.2026

5 · Who and what money

Authors and funding

4 authors.

Abishankari RajkumarDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.

Antoine Gaudreau-LapierreDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1H 8M5, Canada. Electronic address: agaud100@uottawa.ca.

Curtis L F AnthonyDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1H 8M5, Canada.

Laura Trinkle-MulcahyDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1H 8M5, Canada. Electronic address: ltrinkle@uottawa.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we provide a protocol for conditional mapping of proximal interactomes of dimeric protein complexes using a BioID-based complementation approach. We describe steps for fusing amino- and carboxyl-terminal fragments of the biotin ligase TurboID to proteins known to interact within cells. This interaction drives re-formation of an active ligase and biotinylation of nearby proteins. We then detail procedures for capturing biotinylated proteins on a streptavidin affinity matrix for identification by mass spectrometry and Python-based scripts for identifying high-confidence proximal interactors. For complete details on the use and execution of this protocol, please refer to Rajkumar et al.

Indexed as

Cell-based AssaysCell BiologyProteomics

Identifiers

PMID42555348

DOI10.1016/j.xpro.2026.104759

PMCPMC13464226

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.