Evidence map›Paper›PMID 42555202›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Sonoselective Transfection of Glioma Endothelium.

Anna C Debski, Catherine M Gorick, Victoria R Breza, Jackson Tirrell, Krishan Perumal, Katherine M Nowak, Ji Song, Natasha D Sheybani, Richard J Price

Abstract read
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In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Anna C DebskiDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Catherine M GorickDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.ORCID https://orcid.org/0000-0002-0139-3438
Victoria R BrezaDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Jackson TirrellDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.ORCID https://orcid.org/0009-0001-7014-7732
Krishan PerumalDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Katherine M NowakDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Ji SongDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.
Natasha D SheybaniDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.ORCID https://orcid.org/0000-0002-1137-058X
Richard J PriceDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, USA.ORCID https://orcid.org/0000-0002-0237-2102

Funding

Genome Editing the Blood-Brain Barrier with Sonoselective Focused UltrasoundR01EB030744 · NIBIB · UNIVERSITY OF VIRGINIA · PI PRICE, RICHARD J. · 2021 to 2024
$2.4M
Focused ultrasound pre-conditioning for augmented nanoparticle penetration in infiltrative gliomasR01EB030409 · NIBIB · UNIVERSITY OF VIRGINIA · PI HANES, JUSTIN S., PRICE, RICHARD J. · 2021 to 2024
$2.3M
MR Image-Guided Drug Delivery to Cerebral Cavernous Malformations with Focused UltrasoundR01CA279134 · NCI · UNIVERSITY OF VIRGINIA · PI Richard J. Price · 2024 to 2026
$1.8M
ImmunoPET Assessment of anti-CD47 Immunotherapy Delivery to Glioblastoma with Focused UltrasoundR21NS118278 · NINDS · UNIVERSITY OF VIRGINIA · PI PRICE, RICHARD J. · 2020 to 2020
$420k
Engineering Focused Ultrasound-Mediated Gene Delivery Platforms for GlioblastomaF31CA295067 · NCI · UNIVERSITY OF VIRGINIA · PI DEBSKI, ANNA COLLEEN · 2024 to 2025
$82k
NCI NIH HHS F31 CA295067NCI NIH HHS R01 CA279134NIBIB NIH HHS R01 EB030409NIBIB NIH HHS R01 EB030744NINDS NIH HHS R21 NS118278UVa Comprehensive Cancer Center
6 · The paper itself

Abstract

The blood-brain and blood-tumor barriers impede therapeutic delivery to glioblastoma (GBM). Furthermore, dysfunctional endothelial cells in GBM enable an immunosuppressive tumor microenvironment (TME) and enhance therapeutic resistance. However, the significance of endothelial dysfunction for GBM progression also positions the endothelium as a rich target for gene therapy. Here, we leveraged the presence of exofacial thiols (SH) on cells within the TME and developed polymeric, plasmid-bearing, densely PEGylated, polyethyleneimine nanoparticles (NPs) functionalized with free thiol groups to facilitate targeting and transfection (SH-NPs). Delivering SH-NPs to GBM with focused ultrasound (FUS) elicited exceptionally high tropism for GBM endothelium (>70% efficiency), significantly surpassing that achieved via convection-enhanced delivery (CED). "Sonoselective," endothelial cell-specific, transfection was then achieved by incorporating the CD144 promoter. Using this system, we delivered a CXCL9 plasmid to GBM endothelium. This intervention enhanced CD8

Indexed as

brain tumorcancer researchendothelial dysfunctionendotheliumfocused ultrasoundgenetic enhancementglioma transfectiontropismtumor microenvironment

Identifiers

PMID42555202
PMCPMC13440367

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.