Evidence map›Paper›PMID 42554480›Full record

ArticleMicrobiology spectrum2026

Whole-genome sequencing of adenovirus 41 directly from wastewater using nested overlapping PCR and MinION.

Naziba Nusrat, John S Meschke, Rachel Swanstrom, Roberto A Rodríguez

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Naziba NusratDepartment of Environmental and Occupational Health Sciences, Bloomington-School of Public Health, Indiana University, Bloomington, Indiana, USA.
John S MeschkeEnvironmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, Washington, USA.
Rachel SwanstromEnvironmental and Occupational Health Sciences, School of Public Health, University of Washington, Seattle, Washington, USA.
Roberto A RodríguezDepartment of Environmental and Occupational Health Sciences, Bloomington-School of Public Health, Indiana University, Bloomington, Indiana, USA.ORCID 0000-0002-0560-6672

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human adenovirus F41 (HAdV-F41) is one of the leading causes of children's acute gastroenteritis and was recently linked to an outbreak of severe acute hepatitis of unknown etiology among children during 2021 to 2022. While most evidence is based on clinical data, wastewater-based epidemiology offers a community-level approach to monitoring circulating strains and enhancing outbreak preparedness. In this study, we developed an overlapping amplicon-based whole-genome sequencing approach to directly detect HAdV-F41 from archived wastewater samples, using nested PCR with 13 primer sets. Archived wastewater samples were collected between 2021 and 2022 from three treatment plants in Seattle, USA. The viral load ranged from 1.2 × 10

Indexed as

Adenoviruses, HumanWastewaterWhole Genome SequencingDNA, ViralGastroenteritisGenome, ViralHumansPhylogenyPolymerase Chain ReactionWashingtonDNA, ViralWastewaterenteric adenoviruswastewater-based epidemiologywhole-genome sequencing

Identifiers

PMID42554480
PMCPMC13532293

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.