Evidence map›Paper›PMID 42553565›Full record

ArticlemicroPublication biology2026

3-iodotyrosine fluorescent proteins for improved expansion microscopy.

Will Scott, Nimo Abdullah, Ragnheiður Guðjónsdóttir, Teresa Massam-Wu, Maria Denisova, Corenna Smith, Falk Schneider, Karuna Sampath, Masanori Mishima, Mohan Balasubramanian

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In one paragraph

Article in microPublication biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Will Scott *Centre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, University of Warwick, Coventry, ENG, United Kingdom.
Nimo Abdullah *Centre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, University of Warwick, Coventry, ENG, United Kingdom.
Ragnheiður GuðjónsdóttirCentre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, University of Warwick, Coventry, ENG, United Kingdom.
Teresa Massam-WuCentre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, University of Warwick, Coventry, ENG, United Kingdom.
Maria DenisovaCentre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, University of Warwick, Coventry, ENG, United Kingdom.
Corenna SmithCentre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, University of Warwick, Coventry, ENG, United Kingdom.
Falk SchneiderCellular Interfaces Cluster, Centre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, University of Warwick, Coventry, ENG, United Kingdom.
Karuna SampathCentre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, University of Warwick, Coventry, ENG, United Kingdom.
Masanori MishimaCentre for Mechanochemical Cell Biology, Warwick Medical School, University of Warwick, University of Warwick, Coventry, ENG, United Kingdom.
Mohan BalasubramanianCentre for Mechanochemical Cell Biology, Division of Biomedical Sciences, Warwick Medical School, University of Warwick, University of Warwick, Coventry, ENG, United Kingdom.

Funding

Wellcome Trust
6 · The paper itself

Abstract

Expansion microscopy is a powerful technique for achieving nanoscale resolution, but labelling and detection using fluorescent proteins (FPs) have been hampered by signal degradation during expansion. Current approaches therefore rely heavily on immunostaining, ssDNA-FISH or HALO/SNAP-based detection systems. Here we show that substituting tyrosine with 3-iodotyrosine in the chromophore of superfolder GFP dramatically improves signal retention during expansion microscopy in zebrafish embryos. This enhancement specifically protects FPs during the formaldehyde-dependent anchoring step, and may additionally reflect better tolerance of the prolonged acidic conditions encountered during anchoring. 3-iodotyrosine-modified FPs open a door to reliable protein-based expansion microscopy.

Identifiers

PMID42553565
PMCPMC13435085

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.