TaqMan-probe-based multiplex real-time qPCR assay for simultaneous detection of Duck Tembusu virus, Novel duck reovirus, and Duck circovirus.

Zhixian Wang, Haoran Xi, Jia Tan, Cui Lin, Xiaona Gao, Siyi Wang, Haiqin Li, Xiaoquan Guo

Abstract read

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Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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5 · Who and what money

Authors and funding

8 authors.

Zhixian Wang *Jiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.

Haoran Xi *Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, China.

Jia TanInstitute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, China.

Cui LinInstitute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, China.

Xiaona GaoJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.

Siyi WangJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.

Haiqin LiInstitute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, China.

Xiaoquan GuoJiangxi Provincial Key Laboratory for Animal Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Duck Tembusu virus (DTMUV), Novel duck reovirus (NDRV), and Duck circovirus (DuCV) are the primary epidemic pathogens threatening China's waterfowl farming industry. The immunosuppressive characteristics of these viruses readily facilitate mixed infections between pathogens, thereby increasing the difficulty of clinical diagnosis and disease prevention and control. To achieve rapid simultaneous detection of these three pathogens, this study designed specific primers and probes based on conserved viral regions, successfully establishing a TaqMan multiplex real-time quantitative PCR assay. The results demonstrate that the method established in this study exhibits excellent specificity and shows no cross-reactivity with other pathogens in waterfowl. The limit of detection (LOD) of this method for the three pathogens was 10

Indexed as

Duck circovirusDuck Tembusu virusmultiplex real-time qPCRNovel duck reovirusTaqMan

Identifiers

PMID42553291

DOI10.3389/fmicb.2026.1884322

PMCPMC13433525

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.