Evidence map›Paper›PMID 42552792›Full record

ArticleMicrobiologyOpen2026

MetF-Dependent Methionine Biosynthesis Is Required for Mycobacterium tuberculosis Survival and In Vivo Persistence.

Ikue Tosa, Tomoki Kitahara, Katsuki Takebe, Masaaki Nakayama, Yuko Ito, Akihito Nishiyama, Shintaro Seto, Hotaka Kawai, Hiharu Inoue, Yuki Fukada and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MicrobiologyOpen, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Ikue TosaDepartment of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Tomoki KitaharaDepartment of Bacteriology, School of Medicine, Niigata University, Niigata, Japan.
Katsuki TakebeDepartment of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.ORCID https://orcid.org/0000-0002-1611-1042
Masaaki NakayamaDepartment of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Yuko ItoDepartment of Bacteriology, School of Medicine, Niigata University, Niigata, Japan.
Akihito NishiyamaDepartment of Bacteriology, School of Medicine, Niigata University, Niigata, Japan.
Shintaro SetoDepartment of Pathophysiology and Host Defense, The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Tokyo, Japan.
Hotaka KawaiDepartment of Oral Pathology and Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Hiharu InoueGraduate School of Human Life and Ecology, Osaka Metropolitan University, Osaka, Japan.
Yuki FukadaDepartment of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Yuki NishiyaDepartment of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Hitoshi NagatsukaDepartment of Oral Pathology and Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Takayuki WadaGraduate School of Human Life and Ecology, Osaka Metropolitan University, Osaka, Japan.
Manabu AtoLeprosy Research Center, National Institute of Infectious Diseases, Japan Institute for Health Security, Tokyo, Japan.
Sohkichi MatsumotoDepartment of Bacteriology, School of Medicine, Niigata University, Niigata, Japan.
Naoya OharaDepartment of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.ORCID https://orcid.org/0000-0003-3486-2373

Funding

Japan Agency for Medical Research and Development 21fk0108090h0003Japan Agency for Medical Research and Development 25gm1610009h0004Japan Agency for Medical Research and Development JP223fa627005Japan Society for the Promotion of Science JP24K02277Japan Society for the Promotion of Science JP24K02614
6 · The paper itself

Abstract

Methionine biosynthesis is essential for Mycobacterium tuberculosis, but the in vivo relevance of individual enzymes remains unclear. We performed transposon sequencing in Mycobacterium bovis BCG under methionine-free conditions and identified metF as a major fitness determinant. To define enzyme-specific functions, ΔmetE and ΔmetF mutants were generated in M. tuberculosis and evaluated using in vitro growth assays and a murine infection model. The ΔmetE mutant showed vitamin B

Indexed as

Bacterial ProteinsMethionineMicrobial ViabilityMycobacterium tuberculosisAnimalsDisease Models, AnimalLungMiceMycobacterium bovisSpleenTuberculosisVitamin B 12Bacterial ProteinsMethionineVitamin B 12bacterial growthin vivo essentialityMetFmethionine biosynthesis pathwaymouse infection modelmycobacterium tuberculosis

Identifiers

PMID42552792
PMCPMC13438895

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.