Evidence map›Paper›PMID 42552309›Full record

ArticleNature communications2026

Human wastewater contamination drives the emergence of multidrug-resistant bacteria in the Galápagos marine ecosystem.

Arnav Lal, Jade C Riopelle, Katherine Villarin, Maya Mathur, Lia Enriquez, Rui Xiao, Naomi Phemister-Jimenez, Katherine Gilbert, Stephen D Cole, Maddie Tilyou and 6 more

Abstract read
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Arnav LalDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.ORCID 0000-0002-1653-4184
Jade C RiopelleDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Katherine VillarinDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Maya MathurDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Lia EnriquezGalápagos Education and Research Alliance, Penn Global Research Institute, University of Pennsylvania, Philadelphia, PA, USA.
Rui XiaoDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Naomi Phemister-JimenezDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Katherine GilbertDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Stephen D ColeDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Maddie TilyouDepartment of Philosophy, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, USA.
Kelly P KennedyDepartment of Philosophy, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, USA.
Ernesto VacaParque Nacional Galápagos, Isla San Cristóbal, Galápagos, Ecuador.
Wilson CastilloParque Nacional Galápagos, Isla San Cristóbal, Galápagos, Ecuador.
Michael WeisbergDepartment of Philosophy, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, USA.
Lisa M MatteiDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA. lmattei@upenn.edu.ORCID 0000-0002-2238-0281
Daniel P BeitingDepartment of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA. beiting@upenn.edu.ORCID 0000-0002-2865-4589

Funding

National Science Foundation (NSF) STS-1557138
6 · The paper itself

Abstract

Antimicrobial resistance poses a global threat to public health. Mobile microbiological laboratories can enable environmental monitoring of antimicrobial resistance, particularly in geographically remote and resource-limited locations, such as the Galápagos archipelago. Here, we report the development of a mobile laboratory for antimicrobial resistance surveillance of marine sites surrounding San Cristóbal, the archipelago's second most populated island, which has experienced rapid urbanization and intense international tourism pressure. On-site metagenomic sequencing of wastewater-contaminated marine sites reveals a stark shift in microbial genera and a higher count of antimicrobial resistance genes compared to uncontaminated marine sites, mirroring metagenomic results of local untreated sewage. Over 40% of lactose-fermenting Enterobacteriaceae isolates collected directly from sewage or marine environments near sites of wastewater outfall exhibit multidrug resistance. Long-read sequencing and de novo assembly of bacterial genomes and plasmids from multidrug-resistant Escherichia coli reveal frequent and rapid reassortment of antimicrobial resistance genes on plasmids, generating a diverse and functional resistome on the island. This study not only provides a framework for conducting antimicrobial resistance research in low-resource settings but also underscores the impact of wastewater contamination on the environmental antimicrobial resistance landscape and highlights potential threats to human and animal health.

Indexed as

BacteriaDrug Resistance, Multiple, BacterialWastewaterAnti-Bacterial AgentsEcosystemEcuadorEnterobacteriaceaeEscherichia coliHumansMetagenomicsPlasmidsSeawaterSewageAnti-Bacterial AgentsSewageWastewater

Identifiers

PMID42552309
PMCPMC13439418

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.