ArticleJournal of veterinary science2026
Optimized
Article in Journal of veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
importanceNatural killer (NK) cells are critical effectors of innate immune surveillance and can eliminate malignant cells without prior sensitization. Given the biological and genetic parallels between canine and human cancers, NK cell-based immunotherapies have strong translational potential in veterinary oncology. However, the lack of an efficient and reproducible
objectiveThis study aimed to evaluate the effectiveness of genetically modified feeder cells and optimized culture media in expanding canine NK cells.
methodsCanine peripheral blood mononuclear cells were co-cultured with irradiated genetically modified K562 or ARH77 cells in either RPMI-1640 or Dulbecco's Modified Eagle Medium (DMEM)/F12-based media. NK cell expansion, purity, cytotoxicity and expression of NK cell-associated receptors were evaluated using flow cytometry, cytotoxicity assays, and quantitative reverse transcription polymerase chain reaction.
resultsThe combination of DMEM/F12-based medium and genetically modified ARH77 feeder cells yielded the highest NK cell expansion rate and purity among the tested conditions. Cytotoxicity differed significantly according to medium type, whereas no significant difference was observed between feeder cell types. In addition, the NK cell-associated receptors were significantly upregulated in expanded NK cells. CONCLUSIONS AND RELEVANCE: Genetically modified ARH77 feeder cells with DMEM/F12-based culture media demonstrated superior efficiency in promoting the
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.