3D cell assemblies for controlled evaluation of response to dual agent delivery nanofibers targeting bacterial vaginosis.

Arielle Greiner, Anthony J Kyser, Hermann B Frieboes

Abstract read

In one paragraph

Article in Biomaterials advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Arielle GreinerDepartment of Bioengineering, University of Louisville Speed School of Engineering, Louisville, KY, 40202, USA.

Anthony J KyserDepartment of Bioengineering, University of Louisville Speed School of Engineering, Louisville, KY, 40202, USA.

Hermann B FrieboesDepartment of Bioengineering, University of Louisville Speed School of Engineering, Louisville, KY, 40202, USA; Center for Predictive Medicine, University of Louisville, Louisville, KY, 40202, USA; Department of Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY, 40202, USA; UofL Health - Brown Cancer Center, University of Louisville, KY, 40202, USA. Electronic address: hbfrie01@louisville.edu.

Funding

3D-bioprinting of sustained- and phased-release antibiotic and probiotic scaffolds to treat bacterial vaginosisR01AI168475 · NIAID · UNIVERSITY OF LOUISVILLE · PI FRIEBOES, HERMANN, LEWIS, AMANDA L. · 2022 to 2025

$3.0M

Understanding the Host-Microbiome-Therapeutic Triad: Implications for Designing Alternative Intravaginal Delivery Platforms to Treat Bacterial VaginosisR01AI139671 · NIAID · UNIVERSITY OF LOUISVILLE · PI FRIEBOES, HERMANN, LEWIS, AMANDA L. · 2019 to 2023

$2.5M

NIAID NIH HHS R01 AI139671NIAID NIH HHS R01 AI168475

6 · The paper itself

Abstract

Inflammatory response to Gardnerella during bacterial vaginosis (BV) is linked to complications including preterm birth and infection susceptibility. Platforms for local antibiotic and probiotic co-delivery have recently emerged to promote user adherence and avoid recurrence. This study designed a novel 3D cell assembly for controlled response evaluation to co-delivery platforms. Vaginal bilayer model was configured with 3.0 μm pore inserts in 24-well plates, with confluent vaginal epithelial cells (VK2 E6/E7) in apical chamber and peripheral mononuclear cells (PBMCs) in basal chamber. PEO (rapid-dissolving) and PEO:PLGA (sustained-release) nanofibers were loaded with 7.5 mg metronidazole and 5x10

Indexed as

Anti-Bacterial AgentsDrug Delivery SystemsMetronidazoleNanofibersProbioticsVaginosis, BacterialCell LineEpithelial CellsFemaleHumansInterleukin-8Lactobacillus crispatusLeukocytes, MononuclearTumor Necrosis Factor-alphaAnti-Bacterial AgentsInterleukin-8MetronidazoleTumor Necrosis Factor-alpha3D co-cultureAntibioticsBacterial vaginosisNAMsNanofibersNew approach methodologiesProbiotics

Identifiers

PMID42551328

DOI10.1016/j.bioadv.2026.215095

PMCPMC13497992

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.