Evidence map›Paper›PMID 42550321›Full record

ArticleFish physiology and biochemistry2026

Sexually dimorphic expression and hormonal responsiveness of steroidogenic Cyp genes during gonadal differentiation in mandarin fish.

Jieming Zhang, Shengyue Lin, Ziyan Deng, Weijian Chen, Guojun Cai, Min Zhang, Lilin Zhan, Chong Han, Jian Gong, Qiang Li

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Article in Fish physiology and biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Jieming Zhang *South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Shengyue Lin *South China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Ziyan DengSouth China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Weijian ChenSouth China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Guojun CaiSouth China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Min ZhangSouth China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Lilin ZhanSouth China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China.
Chong HanSouth China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China. hanch@gzhu.edu.cn.
Jian GongKey Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou, 510006, China. jiangong@gzhu.edu.cn.
Qiang LiSouth China Biodiversity Research Center, School of Life Sciences, Guangzhou University, Guangzhou, 510006, China. bioskylq@gzhu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Steroid hormones play a pivotal role in fish sex differentiation, yet the dynamic expression patterns of key steroidogenic enzymes during this process remain incompletely characterized. Here, we combined genome-wide identification, time series transcriptomes spanning gonadal development (5-360 days post-hatch), and multiple hormone treatment experiments (17α-methyltestosterone, estrone, and etonogestrel) to investigate the Cyp11, Cyp17, Cyp19, and Cyp21 subfamilies in mandarin fish (Siniperca chuatsi). Seven steroidogenic Cyp genes were identified, showing teleost-specific expansion, with one duplicated pair (cyp17a2 and cyp2u1) exhibiting strong purifying selection. Expression profiling revealed pronounced sexually dimorphic and stage-specific patterns: During female differentiation (20-30 days), cyp19a1a and associated genes were highly expressed, coinciding with ovarian differentiation; during male differentiation (30-60 days), cyp17a2 and related genes were upregulated, aligning with testicular development. Exogenous hormone treatments further demonstrated that these genes are dynamically responsive: cyp19a1a and cyp17a2 were highly responsive to androgenic and progestogenic treatments, and their expression changes correlated closely with gonadal sex reversal phenotypes observed histologically. Collectively, this study provides a comprehensive expression atlas of steroidogenic Cyp genes during gonadal differentiation and identifies key hormonally responsive candidates for sex control in aquaculture.

Indexed as

Cytochrome P-450 Enzyme SystemGonadsPerciformesSex DifferentiationAnimalsFemaleGene Expression Regulation, DevelopmentalGene Expression Regulation, EnzymologicMaleMethyltestosteroneSex CharacteristicsCytochrome P-450 Enzyme SystemMethyltestosteroneCypGonadal developmentHormonal regulationMandarin fish (Siniperca chuatsi)Sex differentiationSteroidogenesis

Identifiers

PMID42550321

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.