Integrated DIA-PRM proteomics Deciphers the molecular mechanisms and identifies core biomarkers of relative resistance to decapod iridescent Virus 1 (DIV1) in Macrobrachium rosenbergii.

Yang Xu, Chenhui Wu, Yunpeng Fan, Chaofan Yu, Qiang Gao

Abstract read

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Article in Marine biotechnology (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Yang XuNational Genetic Breeding Center for Macrobrachium rosenbergii, Zhejiang Institute of Freshwater Fisheries (Zhejiang Freshwater Fishery Environmental Monitoring Station), No. 999 Hangchangqiao South Road, Wuxing District, Huzhou, Zhejiang, 313001, China.

Chenhui WuNational Genetic Breeding Center for Macrobrachium rosenbergii, Zhejiang Institute of Freshwater Fisheries (Zhejiang Freshwater Fishery Environmental Monitoring Station), No. 999 Hangchangqiao South Road, Wuxing District, Huzhou, Zhejiang, 313001, China.

Yunpeng FanNational Genetic Breeding Center for Macrobrachium rosenbergii, Zhejiang Institute of Freshwater Fisheries (Zhejiang Freshwater Fishery Environmental Monitoring Station), No. 999 Hangchangqiao South Road, Wuxing District, Huzhou, Zhejiang, 313001, China.

Chaofan YuNational Genetic Breeding Center for Macrobrachium rosenbergii, Zhejiang Institute of Freshwater Fisheries (Zhejiang Freshwater Fishery Environmental Monitoring Station), No. 999 Hangchangqiao South Road, Wuxing District, Huzhou, Zhejiang, 313001, China.

Qiang GaoNational Genetic Breeding Center for Macrobrachium rosenbergii, Zhejiang Institute of Freshwater Fisheries (Zhejiang Freshwater Fishery Environmental Monitoring Station), No. 999 Hangchangqiao South Road, Wuxing District, Huzhou, Zhejiang, 313001, China. fionxu2020@163.com.

Funding

China Agriculture Research System of MOF and MARA CARS-48Ningbo Science and Technology Plan Project 2024Z278

6 · The paper itself

Abstract

Macrobrachium rosenbergii is a pillar species in global freshwater crustacean aquaculture. Decapod iridescent virus 1 (DIV1) causes 60%-90% cumulative mortality in cultured ponds, leading to massive economic losses with no effective control measures. This study aimed to elucidate DIV1 relative resistance mechanisms and screen core biomarkers in M. rosenbergii. We constructed artificially selected DIV1-resistant (GK), susceptible (YG) and control (DZ) group from M. rosenbergii populations. After standardized DIV1 challenge, plasma samples were collected at 72 h post-infection, and analyzed via integrated DIA-MS proteomics, PRM targeted validation and bioinformatics analysis. GK group had a 2.7-fold longer median survival time (118 h vs. 44 h in YG) and 69% lower cumulative mortality risk. The core relative resistance mechanism was identified as enhanced proteostasis network plus selective metabolic reprogramming. PRM validation of 12 core DEPs showed high consistency with DIA. results (Pearson r = 0.92, P < 0.001), with 5 key proteins forming a multi-dimensional antiviral network. This study first reveals a non-canonical anti-DIV1 immune paradigm in crustaceans. The screened core biomarkers provide key molecular targets for M. rosenbergii precision breeding and field early warning, laying a theoretical foundation for sustainable DIV1 control.

Indexed as

PalaemonidaeAnimalsBiomarkersDisease ResistanceProteomeProteomicsBiomarkersProteomeAntiviral mechanismBiomarkerDecapod iridescent virus 1 (DIV1)DIA-PRM integrated proteomicsMacrobrachium rosenbergiiMetabolic reprogramming

Identifiers

PMID42550270

DOI10.1007/s10126-026-10678-3

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.