Evidence map›Paper›PMID 42550083›Full record

ArticleJournal of bacteriology2026

A targeted mutational strategy aiding in generating antisense RNA to knockdown the

Xishuai Tong, Dominica Ferm, Huitao Liu, Ying Wang, Chandramouli Kondethimmanahalli, Roman R Ganta

Abstract read
In one paragraph

Article in Journal of bacteriology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Xishuai Tong *Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.ORCID 0000-0002-2507-4749
Dominica Ferm *Department of Pathobiology and Integrative Biomedical Sciences, Bond Life Sciences Center, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, USA.ORCID 0000-0001-9954-7417
Huitao Liu *Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.ORCID 0000-0002-0769-6712
Ying Wang *Center of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.
Chandramouli KondethimmanahalliCenter of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.ORCID 0000-0002-0104-1993
Roman R GantaCenter of Excellence for Vector-Borne Diseases, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA.ORCID 0000-0001-9794-6158

Funding

Vector and Host Contributions to the Regulation of E. Chaffeensis Gene ExpressionR01AI070908 · NIAID · UNIVERSITY OF MISSOURI-COLUMBIA · PI ROMAN R. GANTA · 2008 to 2026
$6.9M
Genetics and Axenic Growth of tick-borne E. chaffeensis and A. phagocytophilumR01AI182519 · NIAID · UNIVERSITY OF MISSOURI-COLUMBIA · PI ROMAN R. GANTA · 2024 to 2026
$1.6M
NIAID NIH HHS R01 AI070908NIAID NIH HHS R01 AI182519NIH HHS R01AI070908 and R01AI182519
6 · The paper itself

Abstract

Obligate intracellular pathogenic bacteria belonging to the order Rickettsiales include several important emerging pathogens causing major health and economic impact to humans, companion animals, and agricultural animals. Despite some recent progress, the lack of well-established genetic manipulation methods for diverse research applications remains a challenge. We recently reported the establishment of targeted mutagenesis to disrupt genes in IMPORTANCE: We developed an innovative targeted mutagenesis approach in the obligate intracellular bacterium

Indexed as

Bacterial Outer Membrane ProteinsEhrlichia chaffeensisGene Knockdown TechniquesRNA, AntisenseAnimalsGene Expression Regulation, BacterialMiceMutagenesisMutationBacterial Outer Membrane ProteinsRNA, AntisenseAnaplasmataceaeantisense RNAEhrlichia chaffeensismolecular geneticsRickettsialestargeted mutagenesistick-borne pathogen

Identifiers

PMID42550083
PMCPMC13492146

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.