Evidence map›Paper›PMID 42549190›Full record

ArticleChemical engineering journal (Lausanne, Switzerland : 1996)2026

CRISPR-Programmable Nanoparticle Transport in Salt-Gated Porous Media for Visual DNA Detection.

Kurt T Schalper, Rui Yang, Xin Guan, Jiongyu Zhang, Chengyu Hou, Xingye Liu, Minjie Pei, Trystin Cote, Yupeng Chen, Changchun Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical engineering journal (Lausanne, Switzerland : 1996), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kurt T SchalperDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut 06032, United States.
Rui YangDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut 06032, United States.
Xin GuanDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut 06032, United States.
Jiongyu ZhangDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut 06032, United States.
Chengyu HouDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut 06032, United States.
Xingye LiuDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut 06032, United States.
Minjie PeiDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut 06032, United States.
Trystin CoteDepartment of Biomedical Engineering, University of Connecticut, Storrs, Connecticut 06269, United States.
Yupeng ChenDepartment of Biomedical Engineering, University of Connecticut, Storrs, Connecticut 06269, United States.
Changchun LiuDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut 06032, United States.

Funding

Low-Cost CRISPR-on-Paper for Cervical Cancer Screening at the Point of CareU01CA269147 · NCI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI Changchun Liu · 2022 to 2026
$2.6M
Asymmetric CRISPR Approach for Nucleic Acid QuantificationR01EB023607 · NIBIB · UNIVERSITY OF PENNSYLVANIA · PI Changchun Liu · 2017 to 2026
$2.4M
HIV Viral Load Testing Using CRISPR Cascade Signal AmplificationR01AI194917 · NIAID · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI Changchun Liu · 2025 to 2026
$1.3M
NCI NIH HHS U01 CA269147NIAID NIH HHS R01 AI194917NIBIB NIH HHS R01 EB023607
6 · The paper itself

Abstract

Paper-based diagnostics typically treat porous cellulose as a passive capillary medium, with nanoparticle transport governed by channel geometry and capture chemistry. Here we show that CRISPR-programmable nanoparticle surface chemistry can actively regulate transport in paper through a salt-activated adhesion mechanism. Gold nanoparticles functionalized with hydrophobic Cy5-labeled DNA exhibit strong adhesion to cellulose fibers at elevated ionic strength (~1.5 M local NaCl generated upon rehydration of preloaded salt), whereas particles lacking hydrophobic domains migrate freely. This phenomenon enables a distance-based visual readout, in which the length of the colored nanoparticle front provides a semi-quantitative measure of DNA target concentration. Optical and electron microscopy reveal that nanoparticle immobilization arises from extensive fiber adhesion rather than pore occlusion or particle aggregation. As a proof of concept, coupling Cas12a-mediated cleavage of Cy5-labeled DNA probes to this salt-gated transport system enabled naked-eye, distance-based detection of Herpes simplex virus 1 (HSV-1) DNA with a limit of detection of 100 aM in ~45-60 min. Evaluation of 40 clinical samples showed excellent agreement with qPCR, achieving 96.2% sensitivity and 100% specificity. More broadly, this work establishes a general strategy for programming nanoparticle transport in porous media by encoding functionality directly into nanoparticle surface chemistry.

Indexed as

CRISPR-AuNPHSV DetectionPaper microfluidicsSalt-triggered adhesionStimuli-responsive surface chemistry

Identifiers

PMID42549190
PMCPMC13431041

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.