ArticleJournal of tropical medicine2026
Real-Time PCR-Based Detection of
Article in Journal of tropical medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Real-Time PCR-Based Detection ofJournal of tropical medicine · 2026Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Leprosy remains endemic in Côte d'Ivoire, with persistent Grade 2 disability cases indicating ongoing challenges in early diagnosis. Classical diagnosis relies on clinical examination and microscopy, which lacks sensitivity because Methods: We developed and evaluated a real-time PCR protocol targeting the Results: Among 48 samples, microscopy detected only nine (18.8%) positive cases, all from multibacillary (MB) patients. In contrast, real-time PCR detected 35 (72.9%) positives using the NucliSENS kit and 31 (64.6%) using the GuSCN method. In paucibacillary (PB) patients, PCR positivity reached 59.5%, whereas microscopy was nearly negative. Ear lobe exudates provided the best diagnostic yield. Conclusion: Real-time PCR targeting 16S rRNA significantly improves
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