ArticleReproductive medicine and biology
Library Concentration After Whole Genome Amplification Improves Interpretation of Spent Culture Medium-Based Noninvasive PGT-A in Vitrified-Warmed Human Blastocysts.
Article in Reproductive medicine and biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: To evaluate the feasibility and performance of spent culture medium (SCM)-based non-invasive pre-implantation genetic testing for aneuploidy (niPGT-A) after 8 h of post-warming in vitrified-warmed human blastocysts, and to determine whether post-amplification library concentration improves SCM-based result interpretation. Methods: This study included 26 discarded vitrified-warmed blastocysts from 17 patients. After 8 h of post-warming culture, SCM was collected and analyzed by next-generation sequencing together with trophectoderm (TE) biopsy samples and whole blastocysts (WB). Concordance at the chromosome and category levels was evaluated between SCM and WB and between TE biopsy and WB, and the association of library concentration with performance and euploid probability was assessed. Results: SCM analysis was informative in 92.3% (24/26) of embryos. Chromosome-level concordance with WB was lower for SCM than for TE (30.8% vs. 57.7%), whereas category-level concordance was 69.2% for SCM and 76.9% for TE. Combining SCM category with library concentration improved prediction of euploid embryos (AUC 0.9527 vs. 0.8077, Conclusions: SCM-based niPGT-A after 8 h of post-warming culture is feasible in vitrified-warmed human blastocysts. Library concentration improves interpretation of SCM-based results and may help identify embryos likely to be euploid.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.