Evidence map›Paper›PMID 42547220›Full record

ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2026

Chromosomal evolution and speciation inferred from chromosome-scale genome assemblies in three Cupressaceae species.

Kenta Shirasawa, Yuta B Aoyagi, Kentaro Mishima, Hideki Hirakawa, Tomonori Hirao

Abstract read
In one paragraph

Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kenta ShirasawaDepartment of Frontier Research and Development, Kazusa DNA Research Institute, Chiba 292-0818, Japan.ORCID 0000-0001-7880-6221
Yuta B AoyagiDepartment of Frontier Research and Development, Kazusa DNA Research Institute, Chiba 292-0818, Japan.ORCID 0000-0003-2212-1543
Kentaro MishimaKyushu Regional Breeding Office, Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization, Kumamoto 861-1102, Japan.
Hideki HirakawaFaculty of Agriculture, Kyushu University, Fukuoka 819-0395, Japan.ORCID 0000-0002-7128-7390
Tomonori HiraoForest Tree Breeding Center, Forestry and Forest Products Research Institute, Forest Research and Management Organization, Ibaraki 319-1301, Japan.

Funding

JSPS 22H05172JSPS 22H05181Kazusa DNA Research Institute FoundationMAFF JPJ009841
6 · The paper itself

Abstract

Chromosome-scale genome assemblies in gymnosperms have lagged behind those of angiosperms, likely due to their large genomes. Coniferous tree species, which belong to the gymnosperms, are important resources for wood production in the forestry industry. To elucidate the evolution and speciation of these species and establish genome resources for breeding, we integrated draft assemblies with optical and genetic mapping to construct chromosome-scale genomes for Japanese cypress (Chamaecyparis obtusa, 8.7 Gb), Japanese cedar (Cryptomeria japonica, 9.6 Gb), and Chinese fir (Cunninghamia lanceolata, 13.4 Gb). Additionally, we assembled and annotated their chloroplast and mitochondrial genomes. Comparative analysis of the nuclear genomes revealed that while synteny is largely conserved, distinct translocations and inversions occurred in chromosomes 2, 6, and 9. Notably, the significantly larger genome of Cu. lanceolata was associated with frequent tandem gene duplications rather than transposon expansion. These findings suggest that chromosomal rearrangements and segmental duplications played key roles in the divergence of these species. The genomic resources presented here including chromosome-scale sequences, gene annotations, and genetic maps will facilitate advanced conifer genetics and accelerate forest tree breeding programmes.

Indexed as

Chromosomes, PlantCupressaceaeEvolution, MolecularGenetic SpeciationGenome, PlantChromosome MappingGenome, ChloroplastPhylogenySyntenychromosome-scale genome assemblycomparative genomicsCupressaceaeforestrygenetic mapping

Identifiers

PMID42547220
PMCPMC13433000

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.