Evidence map›Paper›PMID 42546586›Full record

ArticleBiomaterials2027

Transient culture of adult human hepatocytes as organoids improves engineered liver engraftment.

Sarah H Saxton, Inkyung Kang, Shannon Mitchell, Jeremy Lotto, Aditya Upadhyay, Chenhui Zou, Corrine Quirk, Tara N McCray, Olivia Prado, Fredrik Johansson and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Sarah H SaxtonDepartment of Bioengineering, University of Washington, Seattle, WA, 98195, USA; Institute for Stem Cell and Regenerative Medicine, Seattle, WA, 98195, USA.
Inkyung KangDepartment of Bioengineering, University of Washington, Seattle, WA, 98195, USA.
Shannon MitchellDepartment of Bioengineering, University of Washington, Seattle, WA, 98195, USA.
Jeremy LottoDepartment of Bioengineering, University of Washington, Seattle, WA, 98195, USA.
Aditya UpadhyayDivision of Gastroenterology and Hepatology, Weill Cornell Medicine, New York, NY, 10065, USA.
Chenhui ZouDivision of Gastroenterology and Hepatology, Weill Cornell Medicine, New York, NY, 10065, USA; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY, 10065, USA.
Corrine QuirkLaboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY, 10065, USA.
Tara N McCrayDepartment of Bioengineering, University of Washington, Seattle, WA, 98195, USA; Institute for Stem Cell and Regenerative Medicine, Seattle, WA, 98195, USA.
Olivia PradoDepartment of Bioengineering, University of Washington, Seattle, WA, 98195, USA; Institute for Stem Cell and Regenerative Medicine, Seattle, WA, 98195, USA.
Fredrik JohanssonDepartment of Bioengineering, University of Washington, Seattle, WA, 98195, USA; Institute for Stem Cell and Regenerative Medicine, Seattle, WA, 98195, USA.
Chelsea L FortinInstitute for Stem Cell and Regenerative Medicine, Seattle, WA, 98195, USA; Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, 98195, USA.
Dorice L GouneDepartment of Bioengineering, University of Washington, Seattle, WA, 98195, USA.
Ekaterini D ZacharisWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, Cambridgeshire, UK.
Ludovic VallierWellcome-MRC Cambridge Stem Cell Institute, Cambridge, Cambridgeshire, UK; Department of Surgery, University of Cambridge and NIHR Cambridge Biomedical Research Centre, Cambridge, Cambridgeshire, UK.
Ype P de JongDivision of Gastroenterology and Hepatology, Weill Cornell Medicine, New York, NY, 10065, USA; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY, 10065, USA.
Kelly R StevensDepartment of Bioengineering, University of Washington, Seattle, WA, 98195, USA; Institute for Stem Cell and Regenerative Medicine, Seattle, WA, 98195, USA; Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY, 10065, USA; Center for Cardiovascular Biology, University of Washington, Seattle, WA, 98195, USA; Brotman Baty Institute, Seattle, WA, 98195, USA. Electronic address: ksteve@uw.edu.

Funding

Toward Safer Gene Therapy for Hemophilia AP01HL160472 · NHLBI · INDIANA UNIVERSITY INDIANAPOLIS · PI Roland W. Herzog · 2022 to 2026
$15.1M
Environmetal Pathology/Toxicology Training ProgramT32ES007032 · NIEHS · UNIVERSITY OF WASHINGTON · PI FAUSTMAN, ELAINE M, ROSENFELD, MICHAEL E · 1985 to 2023
$10.7M
Bioengineering Cardiovascular Training GrantT32EB001650 · NIBIB · UNIVERSITY OF WASHINGTON · PI REGNIER, MICHAEL · 2004 to 2019
$3.6M
Photoabsorbing bioinks for expanding 3D printed human liver in situR01DK128551 · NIDDK · UNIVERSITY OF WASHINGTON · PI STEVENS, KELLY R · 2021 to 2024
$3.0M
Modeling alcohol toxicity in human hepatocytesR01AA027327 · NIAAA · WEILL MEDICAL COLL OF CORNELL UNIV · PI DE JONG, YPE PETER · 2019 to 2023
$1.9M
NHLBI NIH HHS P01 HL160472NIAAA NIH HHS R01 AA027327NIBIB NIH HHS T32 EB001650NIDDK NIH HHS R01 DK128551NIEHS NIH HHS T32 ES007032Wellcome Trust
6 · The paper itself

Abstract

Bioengineered livers could serve as a bridge or alternative to whole organ transplantation for patients suffering from liver disease. However, identifying cellular configurations that promote adult human hepatocyte survival after tissue transplantation remains a critical hurdle. We hypothesized that transient culture of adult hepatocytes in 3D as organoids would enhance engraftment of these cells in bioengineered tissues in vivo. Here, we established an organoid system that supports culture of adult human hepatocytes from eight different human adult donors aged 19-49. These organoids support retention of human hepatocyte morphology found in the mature liver, express an adult liver transcriptome, and exhibit mature functions such as inducible cytochrome activity. Importantly, adult human hepatocyte organoids (AHHO) improved engraftment of hepatocytes up to 25-fold in vivo in engineered tissues compared to previous hepatocyte models. Thus, 'priming' human cells in organoids could have a major impact on translational bioengineered tissue applications.

Indexed as

Cell Culture TechniquesHepatocytesLiverOrganoidsTissue EngineeringAdultAnimalsCells, CulturedFemaleHumansMaleMiddle AgedYoung AdultBioengineered liverHepatocyte organoidsTissue engraftment

Identifiers

PMID42546586
PMCPMC13640435

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.