ArticlePloS one2026
Development of a quantitative PCR assay to detect Gambierdiscus holmesii, a ciguatoxin producing species from Australian waters.
Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A quantitative polymerase chain reaction (qPCR) assay was developed for the species-specific detection and quantification of Gambierdiscus holmesii, recently reported to produce known ciguatoxin (CTX) analogues in north Queensland. The assay was designed to target the ITS region and calibrated using synthetic gBlocks standards and cell-based standard curves to enable accurate quantification. The G. holmesii-specific qPCR assay demonstrated high specificity, efficiency, and sensitivity, with no cross-reactivity observed against closely related Gambierdiscus species. The assay was subsequently applied to environmental samples collected from Heron Island (Great Barrier Reef, Queensland), detecting G. holmesii in 5 out of 12 sites. This newly developed molecular tool enhances the capacity to monitor G. holmesii in the environment and supports improved surveillance of ciguatoxin-producing Gambierdiscus species in Australian waters, enabling earlier detection and more effective management of ciguatera poisoning risks to public health and fisheries.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.