Evidence map›Paper›PMID 42545608›Full record

ArticleProbiotics and antimicrobial proteins2026

Bifidobacterium longum subsp. infantis CCFM1445 Promotes Bone Growth in Growing Mice Via Modulating Gut Microbiota and Arginine Metabolism Pathway.

Mingjie Li, Bowen Li, Haiqin Chen, Qixiao Zhai, Catherine Stanton, R Paul Ross, Jianxin Zhao, Wei Chen, Bo Yang

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Article in Probiotics and antimicrobial proteins, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Mingjie LiState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, China.
Bowen LiState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, China.
Haiqin ChenState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, China.
Qixiao ZhaiState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, China.
Catherine StantonInternational Joint Research Laboratory for Maternal-Infant Microbiota and Health, Jiangnan University, Wuxi, 214122, China.
R Paul RossInternational Joint Research Laboratory for Maternal-Infant Microbiota and Health, Jiangnan University, Wuxi, 214122, China.
Jianxin ZhaoState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, China.
Wei ChenState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, China.
Bo YangState Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, China. bo.yang@jiangnan.edu.cn.

Funding

Research Program of State Key Laboratory of Food Science and Resources, Jiangnan University No. SKLF-ZZB-202510
6 · The paper itself

Abstract

Bifidobacterium has been shown to regulate bone metabolism and maintain bone homeostasis. This study investigated the effects of Bifidobacterium longum subsp. infantis on bone growth in juvenile mice. Treatment with B. longum subsp. infantis CCFM1445 increased femur length, bone volume fraction, and cortical bone area in both female and male mice. Bone histomorphometry indicated that CCFM1445 elevated the height of the femoral growth plate, increased osteoblast numbers, and decreased osteoclast numbers. Furthermore, B. longum subsp. infantis CCFM1445 raised serum concentrations of bone formation markers, including osteoprotegerin (OPG), procollagen type I N-terminal propeptide (PINP), and bone alkaline phosphatase (BALP). Concurrently, it suppressed the levels of tartrate-resistant acid phosphatase type 5b (TRACP5b) and cross-linked N-telopeptide of type I collagen (NTX). Additionally, CCFM1445 upregulated the transcriptional network of key genes involved in osteogenesis and bone matrix synthesis. And intervention with CCFM1445 also significantly increased the relative abundance of Bifidobacterium, Alistipes, and [Eubacterium] xylanophilum group in the gut microbiota. Targeted metabolomic analysis showed that CCFM1445 modulated the arginine biosynthesis and metabolic pathway, characterized by decreased citrulline and increased levels of arginine and its downstream metabolites. Together, these results demonstrate that B. longum subsp. infantis CCFM1445 enhances bone formation, suppresses bone resorption, modulates gut microbiota composition, and influences arginine metabolism, thereby promoting longitudinal bone growth and increasing bone mass in growing mice.

Indexed as

ArginineB. longum subsp. infantisBone metabolismGut microbiotaJuvenile mice

Identifiers

PMID42545608

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