Evidence map›Paper›PMID 42545594›Full record

ArticleDaru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences2026

Isoquercitrin suppresses neuronal ferroptosis in ischemic stroke via SIRT1-mediated deacetylation of H3K9 at the RBM15 promoter.

Shichang Cai, Siqi Zhou, Yicheng Wang, Zhen Li, Hongzhu Long, Yiping Chen, Meichun Wu, Xiuping Li

Abstract read
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In one paragraph

Article in Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Shichang Cai *School of Basic Medical Sciences, Hunan University of Medicine, Huaihua, China.
Siqi Zhou *School of Nursing, Hunan University of Medicine, Huaihua, China.
Yicheng Wang *College of Traditional Chinese Medicine, Hunan University of Medicine, Huaihua, China.
Zhen LiSchool of Medical Information Engineering, Hunan University of Medicine, Huaihua, China.
Hongzhu LongSchool of Nursing, Hunan University of Medicine, Huaihua, China.
Yiping ChenSchool of Basic Medical Sciences, Hunan University of Medicine, Huaihua, China.
Meichun WuCollege of Chemistry and Materials Engineering, Huaihua University, Huaihua, China. cq0078@163.com.
Xiuping LiSchool of Basic Medical Sciences, Hunan University of Medicine, Huaihua, China. lixiuping0801@hnmu.edu.cn.

Funding

National College Students Innovation and Entrepreneurship Training Program No. S202512214004Pilot and Innovative Program for College Students in Hunan Province Education Department No. S202512214029the Scientific Research Fund of Hunan Provincial Education Department No.24A0762
6 · The paper itself

Abstract

backgroundIsoquercitrin (ISO), a neuroprotective flavonoid, alleviates ischemic stroke (IS) by inhibiting ferroptosis. In present study, we explored the mechanism of ISO attenuates neuronal ferroptosis in IS.

methodsHT22 cells and primary neurons underwent oxygen-glucose deprivation/reoxygenation (OGD/R) to mimic in vitro IS. Dual‑luciferase reporter assays detected luciferase activity. H3K9 acetylation levels at the RBM15 promoter were assessed by ChIP. The m6A modification of SLC25A28 mRNA was analyzed by MeRIP. The binding of RBM15 and IGF2BP3 to SLC25A28 mRNA was examined via RIP. Lipid and intracellular ROS levels were measured by C11-BODIPY and DCFH-DA staining. The levels of ferroptosis-related indicators were determined with commercial kits. Cell viability was examined using the CCK-8 assay. In vivo, the middle cerebral artery occlusion (MCAO) rat model was used to explore the effect of ISO.

resultsISO protected against OGD/R-induced ferroptosis and restored viability. ISO inhibited neuronal ferroptosis by reducing RBM15 expression. ISO suppressed RBM15 via SIRT1-mediated H3K9 deacetylation. Subsequently, RBM15 promoted m6A modification of SLC25A28 mRNA to enhance its stability. Reversal of ISO-mediated ferroptosis suppression by SLC25A28 overexpression was partly counteracted by RBM15 knockdown. In the MCAO model, ISO reduced infarct volume, lowered MDA levels, and restored GPX4 expression, which were partly reversed by RBM15 overexpression.

conclusionISO promoted SIRT1-mediated H3K9 deacetylation at the RBM15 promoter to suppress its transcription. RBM15 downregulation inhibited SLC25A28 stability by m6A-IGF2BP3-dependent, ultimately attenuating neuronal ferroptosis in IS.

Indexed as

FerroptosisIschemic StrokeNeuronsNeuroprotective AgentsQuercetinRNA-Binding ProteinsSirtuin 1AcetylationAnimalsCell LineCell SurvivalHistonesMaleMicePromoter Regions, GeneticRatsHistonesisoquercitrinNeuroprotective AgentsQuercetinRNA-Binding ProteinsSirt1 protein, mouseSirt1 protein, ratSirtuin 1FerroptosisHistone deacetylationIschemic strokeIsoquercitrinRBM15

Identifiers

PMID42545594
PMCPMC13433688

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.