ArticleDoklady. Biochemistry and biophysics2026
Starch as a Polymer-Based Medium Modifier to Analyze Enzyme Activity under Conditions Close to Intracellular Media: Butyrylcholinesterase as a Model Enzyme.
Article in Doklady. Biochemistry and biophysics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors.
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Abstract
The study demonstrates the possibility of using starch to model macromolecular crowding and intracellular medium. A kinetic approach is used to reveal specific functioning of butyrylcholinesterases from different sources in the starch medium. In the study solutions, the Michaelis constant increases, indicating a decrease in the enzyme affinity for the substrate in the presence of the polymer. In the 9% starch solution, this parameter for butyrylcholinesterase from equine serum is 4.6 times higher than in the buffer solution, while the decrease in V
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