ArticleMethods in molecular biology (Clifton, N.J.)2026
Chemoselective Histidine Bioconjugation Using Thiophosphorodichloridate Reagents.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Chemoselective bioconjugation techniques are essential tools for precise modification and functional analysis of proteins. Here, we describe a detailed protocol for histidine-specific labeling using thiophosphorodichloridate reagents, forming stable thiophosphoramidate linkages. The procedure includes synthesis of the labeling reagents, protein modification, purification by ion-exchange chromatography, and subsequent copper-catalyzed azide-alkyne cycloaddition (CuAAC) for further conjugation.
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