ArticleMethods in molecular biology (Clifton, N.J.)2026
Rapid and Wash-free Live Cell Labeling with BenzoTag.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Self-labeling proteins (SLPs) enable the site-specific labeling of genetically expressed proteins with synthetic tags. BenzoTag was evolved from the commonly use self-labeling protein HaloTag7, for improved labeling kinetics with the fluorogenic dye, Bz1. This developed Bz1•BenzoTag conjugate was successfully applied in wash-free live cell imaging of intracellularly expressed BenzoTag and shown to have orthogonal reactivity against the HaloTag7•CA-JF
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