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ArticleActa parasitologica2026

Characterization and Evaluation of Justicia adhatoda L. and Its Green Synthesized Nanoparticles for Its Efficacy in Culex quinquefasciatus.

Vikas Chandra Roy, Prince Leeban Johnwilmet, Rajalakshmi Anbalagan, Jayalakshmi Krishnan, Jefrillah Jebaseelan, Anand Setty Balakrishnan

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Article in Acta parasitologica, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Vikas Chandra RoyDepartment of Epidemiology and Public Health, Central University of Tamil Nadu, Thiruvarur, India.
Prince Leeban JohnwilmetDepartment of Epidemiology and Public Health, Central University of Tamil Nadu, Thiruvarur, India.
Rajalakshmi AnbalaganDepartment of Biotechnology, School of Integrative Biology, Central University of Tamil Nadu, Thiruvarur, India.
Jayalakshmi KrishnanDepartment of Biotechnology, School of Integrative Biology, Central University of Tamil Nadu, Thiruvarur, India.
Jefrillah JebaseelanDepartment of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, India.
Anand Setty BalakrishnanDepartment of Epidemiology and Public Health, Central University of Tamil Nadu, Thiruvarur, India. anandsb@acad.cutn.ac.in.

Funding

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6 · The paper itself

Abstract

backgroundJusticia adhatoda L., commonly referred to as Vasa or Vasaka, is a significant therapeutic shrub in traditional ayurvedic practice. This study examines the therapeutic potential of leaves from J. adhatoda in treating Mosquito-borne diseases. We hypothesized that J. adhatoda extract and its green synthesized silver nanoparticles may have antibacterial and antilarvicidal effects.

methodsThe aqueous leaf extracts of J. adhatoda were employed as a reducing and capping agent for the AgNP synthesis. The synthesized AgNPs were confirmed using Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), UV-Visible Absorbance Spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR). The analysis of plant-derived compounds in the leaf extracts using methanol, ethanol, chloroform and water showed the existence of alkaloids, terpenoids, phenols, tannins and glycosides. The leaf extracts of J. adhatoda and their silver nanoparticles demonstrated significant antibacterial efficacy against S. epidermidis, a bacterium known to cause secondary infections, in the case of Lymphatic Filariasis, antioxidant activity was assessed to characterize the leaf's phytochemical profile and larvicidal efficacy was evaluated against Cx. quinquefasciatus.

resultsPhytochemical analysis showed the presence of alkaloids, terpenoids, phenols, tannins, and glycosides across the solvents. Both the leaf extract and its AgNPs showed significant antibacterial efficacy against S.epidermidis and acted as a good Radical Scavenging agent. The synthesized silver nanoparticles exhibited a better larvicidal effect [LC

conclusionsThe findings highlight the potential of J. adhatoda and its green-synthesised AgNPs as an eco-friendly and promising therapeutic agent in the control and management of larvicidal activity against Cx. quinquefasciatus and antibacterial efficacy against S. epidermidis. More comprehensive studies will be carried out to analyze the efficacy of J. adhatoda and its nanomaterial by evaluating the larvicidal and antibacterial activity by conducting in vitro analysis.

Indexed as

CulexInsecticidesJusticiaMetal NanoparticlesPlant ExtractsAnimalsAnti-Bacterial AgentsAntioxidantsGreen Chemistry TechnologyLarvaMicrobial Sensitivity TestsPlant LeavesSilverSpectroscopy, Fourier Transform InfraredStaphylococcus epidermidisAnti-Bacterial AgentsAntioxidantsInsecticidesPlant ExtractsSilverAnti-bacterialAnti-oxidantCulex quinquefasciatusJusticia adhatoda L.Larvicidal activityPhytochemical screeningSilver nanoparticles

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