Evidence map›Paper›PMID 42541595›Full record

ArticleAngiogenesis2026

Semi-automated quantification of total and avascular retinal area in the rat model of oxygen-induced retinopathy.

Husaam Qureishy, Amelia Morikuni, Michael D Evans, Rehan Krishnan, Ellen C Ingolfsland

Abstract readLetter
PubMed Publisher
In one paragraph

Article in Angiogenesis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Husaam Qureishy *Department of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA.
Amelia Morikuni *Department of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA.
Michael D EvansClinical and Translational Science Institute, University of Minnesota, Minneapolis, MN, USA.ORCID http://orcid.org/0000-0001-7449-3993
Rehan KrishnanDepartment of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA.
Ellen C IngolfslandDepartment of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA. eingolf@umn.edu.ORCID https://orcid.org/0000-0002-0940-9940

Funding

University of Minnesota Clinical and Translational Science Institute (UMN CTSI)UM1TR004405 · NCATS · UNIVERSITY OF MINNESOTA · PI Bruce R Blazar, Damien A Fair · 2023 to 2026
$30.8M
NCATS NIH HHS K12TR004373 and 1UM1TR004405-01A1NCATS NIH HHS UM1TR004405
6 · The paper itself

Abstract

Quantification of retinal and avascular areas in the rat oxygen induced retinopathy (OIR) model is traditionally performed using time-intensive manual methods that may be subject to inter-rater variability. We developed and validated two open-source, semi-automated Fiji (Fiji is Just ImageJ) macros to measure total retinal area (RA) and avascular area (AA) in rat 50/10 OIR retinal flat mounts. Concordance correlation coefficients demonstrated high agreement between macro-derived and manual measurements for RA (0.988), AA (0.985), and AA/RA (0.983), with minimal bias observed. While inter-user variability was higher for macro-based AA measurements compared to manual methods, averaging results from two users improved precision. These semi-automated tools reduce analysis time and training burden while maintaining strong agreement with gold standard measurements. Although limited by user input requirements and single-laboratory validation, these macros provide accessible, standardized methods for retinal quantification and have the potential to improve reproducibility and efficiency in OIR research.

Indexed as

Image Processing, Computer-AssistedOxygenRetinaRetinal DiseasesAnimalsAutomationDisease Models, AnimalRatsReproducibility of ResultsOxygenAvascular areaFlat mountImage analysisOIR

Identifiers

PMID42541595

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.