Evidence map›Paper›PMID 42541430›Full record

ArticleMolecular microbiology2026

The Role of torS and torT Transcription in Driving a Bet-Hedging Strategy for Trimethylamine N-Oxide Reductase Expression in Escherichia coli.

Erin L Mettert, Jeffrey N Carey, Wilma Ross, Mark Goulian, Patricia J Kiley

Abstract read
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In one paragraph

Article in Molecular microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Erin L MettertDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, USA.ORCID https://orcid.org/0000-0001-5361-3474
Jeffrey N CareyGraduate Group in Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.ORCID https://orcid.org/0000-0002-5105-0265
Wilma RossDepartment of Bacteriology, College of Agriculture and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Mark GoulianGraduate Group in Biochemistry and Molecular Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.ORCID https://orcid.org/0000-0003-0076-023X
Patricia J KileyDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, USA.ORCID https://orcid.org/0000-0002-8771-1782

Funding

MEDCHANISM, ACTIVATION, &CONTROL OF RRNA TRANSCRIPTIONR01GM037048 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI GOURSE, RICHARD L. · 1986 to 2020
$5.9M
Regulation of Bacterial Two-Component Signaling by Small Membrane ProteinsR01GM080279 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI GOULIAN, MARK D · 2007 to 2020
$3.7M
Phosphorelay signaling and regulation in bacteriaR35GM139541 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI GOULIAN, MARK D · 2021 to 2025
$2.0M
Bacterial anaerobic iron homeostasisR01GM115894 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI KILEY, PATRICIA J · 2015 to 2018
$1.2M
NIGMS NIH HHS R01 GM037048NIGMS NIH HHS R01 GM080279NIGMS NIH HHS R01 GM115894NIGMS NIH HHS R35 GM139541NIH HHS R01GM080279NIH HHS R01GM115894NIH HHS R01GM37048NIH HHS R35GM139541
6 · The paper itself

Abstract

In Escherichia coli, cell-to-cell variability in aerobic expression of trimethylamine N-oxide (TMAO) reductase serves as a bet-hedging strategy, enabling a subset of cells to continue growing upon a shift to anaerobic conditions in the presence of TMAO. This variability has been attributed to the low abundance of TorS and TorT signaling proteins, which along with TorR, regulate transcription of torCAD encoding TMAO reductase. Here, we characterize the divergent promoters that drive expression of the bipartite sensor and kinase, TorT and TorS. In vitro transcription analysis identified two transcription start sites for torS and one start site for torT, all located in close proximity. Notably, the RNA polymerase-σ

Indexed as

Escherichia coliEscherichia coli ProteinsOxidoreductases, N-DemethylatingDNA-Directed RNA PolymerasesGene Expression Regulation, BacterialMethylaminesPromoter Regions, GeneticTranscription FactorsTranscription, GeneticTranscription Initiation SiteDNA-Directed RNA PolymerasesEscherichia coli ProteinsIscR protein, E coliMethylaminesOxidoreductases, N-DemethylatingTranscription Factorstrimethylamine dehydrogenasetrimethyloxaminebet‐hedgingdivergent promotersIscRregulationTMAO reductiontranscription

Identifiers

PMID42541430
PMCPMC13644379

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.