ArticleBiotechnology reports (Amsterdam, Netherlands)2026
Biochemical and functional benchmarking of plant protein hydrolysates in serum-reduced adherent CHO-K1 cell culture.
Article in Biotechnology reports (Amsterdam, Netherlands), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Chinese Hamster Ovary (CHO) cells are used for therapeutic protein production. Although industrial platforms rely on serum-free media, fetal bovine serum (FBS) remains common in research and development. Reducing serum while maintaining cell growth is of interest. In this study, CHO-K1 cells were adapted from 10% to 1% FBS in Ham's F-12 medium. Six plant protein hydrolysates (wheat, soy, pea, broad bean, rice, and a rice-pea blend) were biochemically characterized and evaluated for their effects on cell proliferation under serum-reduced conditions. CHO-K1 cells tolerated adaptation to 1% FBS, although proliferation decreased compared to 10% FBS. Supplementation with hydrolysates improved cell growth in a concentration-dependent but nonlinear manner, with low concentrations more favorable than higher concentrations. Wheat (W106) and broad bean (F120) peptones showed the strongest effects, partially restoring proliferation toward high-serum conditions. This study provides an evaluation of plant hydrolysates and supports their use as supplements in serum-reduced CHO-K1 culture.
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