Evidence map›Paper›PMID 42539121›Full record

ArticlebioRxiv : the preprint server for biology2026

Elicitation of stem-directed antibodies in rhesus macaques by a conventional hemagglutinin immunogen.

Shuqin Gu, Joel Finney, Kan Luo, Sarah M Valencia, Dieter Mielke, Tarra A Von Holle, Dawn J Marshall, Robert Parks, Laura L Sutherland, Richard M Scearce and 16 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors.

Shuqin GuInfectious Diseases Division, Department of Pediatrics, Duke University, Durham, NC 27710, USA.ORCID 0000-0002-0347-0097
Joel FinneyLaboratory of Molecular Medicine, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Kan LuoDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Sarah M ValenciaInfectious Diseases Division, Department of Pediatrics, Duke University, Durham, NC 27710, USA.
Dieter MielkeDepartment of Surgery, Duke University School of Medicine, Durham, NC 27710, USA.
Tarra A Von HolleDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Dawn J MarshallDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Robert ParksDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Laura L SutherlandDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Richard M ScearceDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Kevin WieheDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Sampa SantraBeth Israel Deaconess Medical Center, Boston, MA, 02215, USA.
Summer HarrisDepartment of Pathology, Duke University School of Medicine, Durham, NC 27710, USA.
Jacquelyn DuVallDepartment of Pathology, Duke University School of Medicine, Durham, NC 27710, USA.
Chelsea D LandonDepartment of Pathology, Duke University School of Medicine, Durham, NC 27710, USA.
M Ariel SpurrierDepartment of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC 27710, USA.
Nicholas S HeatonDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Hadi M YassineBiomedical Research Center- QU health, Qatar University, Doha Qatar.
Barney S GrahamDepartment of Medicine and Microbiology, Biochemistry, & Immunology, Morehouse School of Medicine, Atlanta, GA 30310, USA.
Thomas B KeplerDepartment of Microbiology, Boston University, Boston, MA 02118, USA.
Hua-Xin LiaoDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Aaron G SchmidtRagon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
Guido FerrariDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Barton F HaynesDuke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Stephen C HarrisonLaboratory of Molecular Medicine, Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.ORCID 0000-0003-3627-2553
M Anthony MoodyInfectious Diseases Division, Department of Pediatrics, Duke University, Durham, NC 27710, USA.

Funding

Viral Sequencing CoreU19AI067854 · NIAID · DUKE UNIVERSITY · PI LETVIN, NORMAN L · 2006 to 2011
$290.5M
Virology CoreP01AI089618 · NIAID · BOSTON CHILDREN'S HOSPITAL · PI STEPHEN COPLAN HARRISON · 2011 to 2026
$40.0M
NIAID NIH HHS P01 AI089618NIAID NIH HHS U19 AI067854
6 · The paper itself

Abstract

Because they can bind many strains of influenza, antibodies targeting the hemagglutinin (HA) stem have been attractive targets for vaccine development. Many monoclonal antibodies (mAbs) directed at the HA stem have been isolated from humans, and these mAbs have mediated broad protection in animal models. We describe here HA stem-directed mAbs isolated from rhesus macaques immunized with an "ordinary" H1 HA trimer. All immunized rhesus macaques developed high serum titers with broad reactivity to diverse H1N1 and H5N1 viruses, and 7 isolated mAbs strongly blocked canonical stem antibody CR6261 binding to H1. MAb DH726.1 robustly protected mice from lethal challenge with H1N1 and H5N1 viruses, and cryo-EM showed the binding footprint overlapped that of some human mAbs. These findings suggest that vaccination with the standard, trimeric HA immunogens may be sufficient to elicit stem antibodies at titers adequate to protect against zoonotic H5N1 influenza.

Indexed as

H5N1HA stem antibodyinfluenza vaccinesrhesus macaque

Identifiers

PMID42539121
PMCPMC13419718

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.