Evidence map›Paper›PMID 42539106›Full record

ArticlebioRxiv : the preprint server for biology2026

Post-loading ribonucleic acid into lipid nanoparticle carriers.

Navid Bizmark, David F Amelemah, Satya Nayagam, Sai Nikhil Subraveti, Bumjun Kim, Livia Salvati Manni, Kathleen Wood, Nageshwar Rao Yepuri, Michael Moir, Marina Cagnes and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Navid BizmarkDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0002-1582-9909
David F AmelemahDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.
Satya NayagamDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.
Sai Nikhil SubravetiDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.
Bumjun KimDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0002-2198-5342
Livia Salvati ManniSchool of Chemistry and University of Sydney Nano Institute, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0001-8870-2477
Kathleen WoodAustralian Centre for Neutron Scattering, Australian Nuclear Science and Technology Organization, New Illawarra Road, Lucas Heights, NSW 2234, Australia.
Nageshwar Rao YepuriNational Deuteration Facility, Australian Nuclear Science and Technology Organization, New Illawarra Road, Lucas Heights, NSW 2234, Australia.
Michael MoirNational Deuteration Facility, Australian Nuclear Science and Technology Organization, New Illawarra Road, Lucas Heights, NSW 2234, Australia.
Marina CagnesNational Deuteration Facility, Australian Nuclear Science and Technology Organization, New Illawarra Road, Lucas Heights, NSW 2234, Australia.
Nanzhi ZangSynthetic Molecule Pharmaceutical Sciences, Genentech, Inc., South San Francisco, CA 94080, USA.
Gregory G WarrSchool of Chemistry and University of Sydney Nano Institute, University of Sydney, Sydney, NSW 2006, Australia.ORCID 0000-0002-6893-1253
Robert K Prud'hommeDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0003-2858-0097

Funding

X-ray Scattering Technology CoreP30GM133893 · NIGMS · BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB · PI Vivian Stojanoff · 2019 to 2026
$38.6M
A Simultaneous SAXS/WAXS Detector System for Solving Biological StructuresS10OD012331 · OD · STATE UNIVERSITY NEW YORK STONY BROOK · PI ALLAIRE, MARC · 2012 to 2012
$1.1M
NIGMS NIH HHS P30 GM133893NIH HHS S10 OD012331
6 · The paper itself

Abstract

Lipid nanoparticles (LNPs) are effective carriers for messenger ribonucleic acid (mRNA) delivery in vaccines; however, their reliance on extreme cold-chain storage limits global manufacturing and distribution. Conventional LNPs are formed by rapidly mixing four lipids with mRNA through electrostatic interactions between cationic ionizable lipids and negatively charged nucleic acids, facilitating nucleation and precipitation of mRNA-loaded LNPs. However, this binding also accelerates mRNA degradation, requiring stringent cold storage which limits widespread vaccine deployment. To overcome this limitation, we introduce a

Identifiers

PMID42539106
PMCPMC13419738

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.