Evidence map›Paper›PMID 42539040›Full record

ArticleResearch square2026

Higher-order genome architecture of spermatids guides chromatin assembly in mature sperm.

Mashiat Rabbani, Zachary Apell, Timothy J Parnell, Lindsay Moritz, Sion Kim, Sowmya Srinivasan, Ritvija Agrawal, Alexander Vargo, Peter Orchard, Wenxin Xie and 8 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Mashiat RabbaniDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, 48109.
Zachary ApellDepartment of Biostatistics, University of Michigan, Ann Arbor, MI, 48109.
Timothy J ParnellHuntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 84112.ORCID 0000-0002-3632-3691
Lindsay MoritzDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, 48109.
Sion KimGilbert S. Omenn Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109.
Sowmya SrinivasanDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, 48109.
Ritvija AgrawalDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, 48109.
Alexander VargoDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, 48109.
Peter OrchardDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, 48109.
Wenxin XieDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, 48109.
Lydia FreddolinoGilbert S. Omenn Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109.
Alan P BoyleDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, 48109.
Jun Z LiDepartment of Molecular Genetics and Genome Sciences, University of Oklahoma Health Science Center, College of Medicine, 73019.
Bluma J LeschDepartment of Genetics, Yale School of Medicine, New Haven, CT 06510.
Bradley CairnsHuntsman Cancer Institute, University of Utah School of Medicine, Salt Lake City, UT 84112.ORCID 0000-0002-9864-8811
Minji KimGilbert S. Omenn Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109.ORCID 0000-0002-7282-8224
Thomas E WilsonDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, 48109.
Saher Sue HammoudDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, 48109.ORCID 0000-0002-5040-6370

Funding

The role of ZCWPW1 in meiosisR01GM148028 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Francesca Cole, Saher Sue Hammoud · 2023 to 2026
$2.4M
Contributions of Sperm Chromatin to development: A Myth or Reality?DP2HD091949 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAMMOUD, SAHER SUE · 2016 to 2016
$2.3M
Career Training in Reproductive BiologyT32HD079342 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Suzanne M MOENTER · 2014 to 2026
$2.3M
Mechanisms of sperm chromatin remodeling in vivo and in vitroR01HD113274 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Saher Sue Hammoud · 2023 to 2026
$2.0M
Sperm Chromatin: Implications on organismal development and fertilityR01HD104680 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAMMOUD, SAHER SUE · 2021 to 2024
$1.8M
NICHD NIH HHS DP2 HD091949NICHD NIH HHS R01 HD104680NICHD NIH HHS R01 HD113274NICHD NIH HHS T32 HD079342NIGMS NIH HHS R01 GM148028
6 · The paper itself

Abstract

Differential packaging of vertebrate sperm and egg genomes is evolutionarily conserved for hundreds of millions of years. Unlike oocytes, post-meiotic spermatid nuclei are thought to uniformly replace histones with transition proteins (TNPs) and, ultimately, with protamines, the small non-histone structural proteins. Tracking of endogenously tagged protamines overturns the classical model of paternal chromatin remodeling, revealing a direct histone-to-protamine-1 transition that occurs independently of both TNPs and protamine-2. Stage-resolved chromatin profiling across 26 spermatid datasets reveals that chromatin compaction is a programmed process that requires H4-hyperacetylation but is not instructed by it. Rather, chromatin remodeling is guided by the pre-meiotic 3D-architecture of the spermatid nucleus: i.e., compaction of the A-compartment precedes B-compartment. This compartment-encoded template for sperm nuclear remodeling suggests that male-specific protamine patterns can transmit pre-meiotic structural information to the zygote and may impact embryonic development.

Identifiers

PMID42539040
PMCPMC13419593

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.