Evidence map›Paper›PMID 42538963›Full record

ArticlebioRxiv : the preprint server for biology2026

A novel multicellular model of the adult mouse sinoatrial node retains spontaneous electrical activity and enables live investigation of the S100B-associated cell population.

Georgiana Luisa Baca, Robert E Monticone, Bruce D Ziman, Shah Md Toufiqur Rahman, Pathik Parekh, Sadia Afrin, Christopher Dunn, Richard Telljohann, Dongmei Yang, Kwan-Wood Gabriel Lam and 10 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Georgiana Luisa BacaLaboratory of Cardiovascular Sciences, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.ORCID 0000-0002-7986-9256
Robert E MonticoneLaboratory of Cardiovascular Sciences, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Bruce D ZimanLaboratory of Cardiovascular Sciences, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Shah Md Toufiqur RahmanLaboratory of Molecular Biology and Immunology, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Pathik ParekhTranslational Gerontology Branch, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Sadia AfrinLaboratory of Genetics and Genomics, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Christopher DunnLaboratory of Molecular Biology and Immunology, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Richard TelljohannLaboratory of Cardiovascular Sciences, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Dongmei YangLaboratory of Cardiovascular Sciences, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Kwan-Wood Gabriel LamLaboratory of Genetics and Genomics, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Peter KilleenTranslational Gerontology Branch, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Dimitrios TsitsipatisLaboratory of Cardiovascular Sciences, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Ana-Maria ZagreanDivision of Physiology-Neuroscience, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.
Myong-Hee SungLaboratory of Molecular Biology and Immunology, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Nigel H GreigTranslational Gerontology Branch, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Supriyo DeLaboratory of Genetics and Genomics, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Allison B HermanLaboratory of Cardiovascular Sciences, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Payel SenLaboratory of Genetics and Genomics, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.ORCID 0000-0003-2809-0901
Rafael de CaboTranslational Gerontology Branch, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.
Edward G LakattaLaboratory of Cardiovascular Sciences, Intramural Research Program, National Institute on Aging, NIH, Baltimore, MD, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Approximately half of the adult sinoatrial node (SAN) consists of non-myocyte populations, indicating that cardiac pacemaking depends on interactions within a multicellular tissue rather than on pacemaker cardiomyocytes alone. Among these, an S100B-associated cell population has been implicated in pacemaker function, yet its identity and physiological roles remain poorly understood. These cells are rare and dispersed throughout the small, structurally complex SAN, making them difficult to observe repeatedly while preserving the native multicellular environment. Here, we established a dissociated multicellular culture of adult mouse SAN tissue on soft collagen-gelatin hydrogels that retains spontaneous electrical activity and permits longitudinal live imaging of S100B-associated cells. Using an S100B-EGFP

Indexed as

cardiac pacemakercellular heterogeneityglial cellslive-cell imagingmicroelectrode arraymulticellular culturenon-myocyte cellsprimary cell cultureS100Bsinoatrial node

Identifiers

PMID42538963
PMCPMC13419512

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.