Evidence map›Paper›PMID 42538915›Full record

ArticlebioRxiv : the preprint server for biology2026

Chemokine receptor activity is differentially regulated by membrane cholesterol.

Fernando Salgado-Polo, Jose Fernández-González, Carolina Ferrera-Mena, Suresh Subedi, Sriram Tiruvadi-Krishnan, Philip Ben Rainsford, Rajesh Regmi, Rajan Lamichhane, Martin Gustavsson

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Fernando Salgado-PoloDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.ORCID 0000-0001-8362-5281
Jose Fernández-GonzálezDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
Carolina Ferrera-MenaDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
Suresh SubediDepartment of Biochemistry & Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee, USA.
Sriram Tiruvadi-KrishnanDepartment of Biochemistry & Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee, USA.ORCID 0000-0002-0423-909X
Philip Ben RainsfordDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.ORCID 0000-0002-0434-4586
Rajesh RegmiDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
Rajan LamichhaneDepartment of Biochemistry & Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee, USA.ORCID 0000-0003-0089-6452
Martin GustavssonDepartment of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.ORCID 0000-0002-3015-2043

Funding

Conformational Dynamics of G Protein-Coupled Receptors at the Single-Molecule LevelR35GM142946 · NIGMS · UNIVERSITY OF TENNESSEE KNOXVILLE · PI LAMICHHANE, RAJAN · 2021 to 2025
$2.2M
NIGMS NIH HHS R35 GM142946
6 · The paper itself

Abstract

Cholesterol is a key membrane component that regulates G protein-coupled receptor (GPCR) function, yet its molecular mechanisms remain unclear. Here, we combine chemical extraction of membrane sterols with functional signaling assays and single-molecule fluorescence resonance energy transfer (smFRET) to define how cholesterol controls activation of chemokine receptors. Reduction of membrane cholesterol in mammalian cells selectively decreased constitutive and agonist-induced signaling across CXCR1, CXCR2, CXCR4, while it activated ACKR3, and did not affect CXCR3, revealing receptor-specific dependence on membrane sterols. Mechanistically, cholesterol regulation partly required the conserved class A GPCR residue Trp

Indexed as

CXCR4CyclodextrinG protein-coupled receptorNanodiscOxysterolReceptor activationSingle-molecule FRET

Identifiers

PMID42538915
PMCPMC13419399

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.