Evidence map›Paper›PMID 42538812›Full record

ArticleG3 (Bethesda, Md.)2026

A pseudo-phased genome assembly for Hemileia vastatrix reveals an isolate-specific chromosomal haploid trisomy.

Peri A Tobias, Richard J Edwards, Jamie Botting, Giullia di Lorenzo, Vera Inácio, Inês Diniz, Maria do Céu Silva, Leonor Guerra-Guimarães, Vitor Várzea, Robert F Park and 1 more

Abstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Peri A TobiasSchool of Life and Environmental Sciences, The University of Sydney, Camperdown, NSW 2006, Australia.ORCID 0000-0002-5645-0161
Richard J EdwardsOcean Genomes Laboratory, Minderoo OceanOmics Centre at University of Western Australia Oceans Institute, the University of Western Australia, Perth, WA 6009, Australia.ORCID 0000-0002-3645-5539
Jamie BottingOcean Genomes Laboratory, Minderoo OceanOmics Centre at University of Western Australia Oceans Institute, the University of Western Australia, Perth, WA 6009, Australia.ORCID 0009-0005-3595-3500
Giullia di LorenzoCentro de Investigação das Ferrugens do Cafeeiro (CIFC), Linking Landscape, Environment, Agriculture and Food (LEAF), Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa 1349-017, Portugal.ORCID 0009-0002-0001-5192
Vera InácioCentro de Investigação das Ferrugens do Cafeeiro (CIFC), Linking Landscape, Environment, Agriculture and Food (LEAF), Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa 1349-017, Portugal.ORCID 0000-0002-5652-2540
Inês DinizCentro de Investigação das Ferrugens do Cafeeiro (CIFC), Linking Landscape, Environment, Agriculture and Food (LEAF), Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa 1349-017, Portugal.ORCID 0000-0003-0193-4385
Maria do Céu SilvaCentro de Investigação das Ferrugens do Cafeeiro (CIFC), Linking Landscape, Environment, Agriculture and Food (LEAF), Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa 1349-017, Portugal.ORCID 0000-0002-1628-7128
Leonor Guerra-GuimarãesCentro de Investigação das Ferrugens do Cafeeiro (CIFC), Linking Landscape, Environment, Agriculture and Food (LEAF), Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa 1349-017, Portugal.ORCID 0000-0002-9676-1036
Vitor VárzeaCentro de Investigação das Ferrugens do Cafeeiro (CIFC), Linking Landscape, Environment, Agriculture and Food (LEAF), Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa 1349-017, Portugal.ORCID 0000-0002-9507-3311
Robert F ParkSchool of Life and Environmental Sciences, Plant Breeding Institute, The University of Sydney, Narellan NSW 2567, Australia.ORCID 0000-0002-9145-5371
Dora BatistaCentro de Investigação das Ferrugens do Cafeeiro (CIFC), Linking Landscape, Environment, Agriculture and Food (LEAF), Associate Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Lisboa 1349-017, Portugal.ORCID 0000-0003-4031-8999

Funding

European Union through FEDER LISBOA-01-0145-FEDER-029189Foundation for Science and Technology PTDC/ASP-PLA/29189/2017Fundação para a Ciência e Tecnologia UID/04129/2025PORLisboaPortugal2020R&D unit LEAF-Linking Landscape, Environment, Agriculture and Food
6 · The paper itself

Abstract

Recurrent epidemics of coffee leaf rust, caused by the fungal pathogen Hemileia vastatrix, have constrained production of Arabica coffee for over 150 years. Here, we present a pseudo-phased, chromosome-level genome resource for H. vastatrix, isolate Hv178a, to guide research into disease management. The Hv178a genome assembly is 665 and 638 Mbp for haplotype A and B, respectively, localized to 18 chromosomes. We determined that the genomes are highly repetitive at ∼94%, with a guanine-cytosine (GC) content of 33.5%. We present the full annotation of 13,760 and 17,998 protein-coding genes, and we predicted 452 and 496 effectors in haplotype A and B, respectively. Depth-based comparisons with 11 additional H. vastatrix isolates revealed increased chromosome 17 (chr17) copy number in Hv178a. qPCR validation supports a chr17 trisomy in Hv178a absent from the ancestral lineage and potentially explaining the observed change in virulence.

Indexed as

BasidiomycotaChromosomes, FungalGenome, FungalHaploidyTrisomyCoffeaHaplotypesMolecular Sequence AnnotationPlant DiseasesCoffea arabicacoffee leaf rustHv178aplant pathogen

Identifiers

PMID42538812
PMCPMC13643790

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.