ArticleBiomedical chromatography : BMC2026
Development and Validation of Simultaneous Quantification of 6TGN and 6MMPN in Whole Blood Using LC-MS/MS: An Application to Therapeutic Drug Monitoring in Hematological Malignancies and in Inflammatory Bowel Diseases.
Article in Biomedical chromatography : BMC, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thiopurine immunosuppressive drugs are indicated in children with acute lymphoblastic leukemia and in both children and adults with chronic inflammatory diseases. Therapeutic drug monitoring (TDM) of its DNA-incorporated metabolites 6-thioguanine nucleotides (6TGN) and 6-methylmercaptopurine nucleotides (6MMPN) is of high interest to optimize efficacy and prevent toxicity. The objective of this study was to develop and validate a sensitive and simple LC-MS/MS method for the simultaneous quantification of 6TGN and 6MMPN in whole blood for TDM. Following red blood cell count and whole blood extraction with dithiothreitol plus acidic hydrolysis at 100°C, quantification was performed using an LC-MS/MS in positive ionization mode. Calibration curves were linear over the range of 20-1000 ng/mL for 6TGN and 20-20,000 ng/mL for 6MMPN, with correlation coefficients above 0.99 for all analytes. Intra- and inter-day precisions were below 9.45%. Extraction recovery and matrix effects reached 78.9%-83.2% and 121% for 6TGN and 92.2%-95.2% and 112%-118% for 6MMPN, respectively. The method was applied to 749 clinical samples from patients with acute lymphoblastic leukemia or chronic inflammatory diseases. A sensitive and simple LC-MS/MS method was validated for 6TGN and 6MMPN in whole blood and was successfully applied to clinical samples for TDM.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.