Evidence map›Paper›PMID 42538352›Full record

ArticleBritish journal of cancer2026

Spatial proteomics deciphers the immune niche landscape of non-ampullary duodenal adenocarcinoma.

Yuichiro Kohara, Satoshi Yasuda, Tomoko Uchiyama, Minako Nagai, Kota Nakamura, Yasuko Matsuo, Takanari Tatsumi, Taichi Terai, Shunsuke Doi, Takeshi Sakata and 5 more

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Article in British journal of cancer, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

15 authors.

Yuichiro KoharaDepartment of Surgery, Nara Medical University, Nara, Japan.
Satoshi YasudaDepartment of Surgery, Nara Medical University, Nara, Japan.
Tomoko UchiyamaDepartment of Diagnostic Pathology, Nara Medical University, Nara, Japan.
Minako NagaiDepartment of Surgery, Nara Medical University, Nara, Japan.
Kota NakamuraDepartment of Surgery, Nara Medical University, Nara, Japan.
Yasuko MatsuoDepartment of Surgery, Nara Medical University, Nara, Japan.
Takanari TatsumiDepartment of Surgery, Nara Medical University, Nara, Japan.
Taichi TeraiDepartment of Surgery, Nara Medical University, Nara, Japan.
Shunsuke DoiDepartment of Surgery, Nara Medical University, Nara, Japan.
Takeshi SakataDepartment of Surgery, Nara Medical University, Nara, Japan.
Yuki OsamuraDepartment of Surgery, Nara Medical University, Nara, Japan.
Sotaro MatsuhisaDepartment of Surgery, Nara Medical University, Nara, Japan.
Akihiko YoshizawaDepartment of Diagnostic Pathology, Nara Medical University, Nara, Japan.
Yutaka SuzukiDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
Masayuki ShoDepartment of Surgery, Nara Medical University, Nara, Japan. m-sho@naramed-u.ac.jp.ORCID http://orcid.org/0000-0002-6208-7661

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNon-ampullary duodenal adenocarcinoma (NADA) is a rare malignancy with an incompletely characterised tumour immune microenvironment (TME). We aimed to elucidate its immune niche landscape and validate a clinically applicable transcriptomic surrogate.

methodsIn 38 resected NADA cases, multiplex immunofluorescence spatial proteomics was performed on tissue microarrays, validated by cohort-wide whole-slide imaging. A 10-gene qRT-PCR panel capturing adaptive immune resistance mechanisms was integrated with spatial data. Molecular microsatellite instability (MSI) testing confirmed mismatch repair (MMR) status in all integrated cases.

resultsSpatial proteomics identified seven distinct cellular niches; the immune-active "Hot spot" niche showed a trend toward prolonged survival. The 10-gene panel classified patients into "Hot," "Intermediate," and "Cold" clusters, with the "Hot" cluster significantly enriched in "Hot spot." A pMMR subgroup with abundant "Hot spot" (7/29, 24.1%) was identified, most belonging to the "Hot" cluster (5/7, 71.4%). Molecular MSI testing confirmed all pMMR "Hot" cases as microsatellite stable. External transcriptomic analysis revealed enrichment of interferon-gamma and inflammatory response pathways in the "Hot" group.

conclusionSpatial proteomics identified an immune-active "Hot spot" niche in NADA. The 10-gene panel serves as a spatial surrogate, identifying a microsatellite-stable pMMR subgroup that may represent a biologically relevant subgroup for future investigation.

Identifiers

PMID42538352

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