Evidence map›Paper›PMID 42537650›Full record

ArticleCell reports methods2026

AAV-delivered TurboRFP enables streamlined tracing and analysis of corticospinal tract sprouting.

Marc Hernaiz-Llorens, Camilo J Londoño, Ellen Wang, Felicia Cai, Junmi M Saikia, Carmine L Chavez-Martinez, Hugo J Kim, Chimuanya K Agba, Binhai Zheng

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In one paragraph

Article in Cell reports methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Marc Hernaiz-LlorensDepartment of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, USA.
Camilo J LondoñoDepartment of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, USA; Neurosciences Graduate Program, University of California San Diego, La Jolla, CA, USA.
Ellen WangDepartment of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, USA; Neurosciences Graduate Program, University of California San Diego, La Jolla, CA, USA.
Felicia CaiDepartment of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, USA.
Junmi M SaikiaDepartment of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, USA; Neurosciences Graduate Program, University of California San Diego, La Jolla, CA, USA.
Carmine L Chavez-MartinezDepartment of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, USA; Biological Sciences PhD Program, University of California San Diego, La Jolla, CA, USA.
Hugo J KimDepartment of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, USA.
Chimuanya K AgbaDepartment of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, USA; Neurosciences Graduate Program, University of California San Diego, La Jolla, CA, USA.
Binhai ZhengDepartment of Neurosciences, School of Medicine, University of California San Diego, La Jolla, CA, USA; VA San Diego Research Service, San Diego, CA, USA. Electronic address: bizheng@health.ucsd.edu.

Funding

U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
Training Program in Basic Clinical GeneticsT32GM008666 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI HAMILTON, BRUCE A · 1998 to 2021
$8.6M
The Role of a Pair of MAP3Ks in the Multicellular Response to Spinal Cord InjuryR01NS093055 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ZHENG, BINHAI · 2015 to 2024
$4.0M
UC San Diego Genetics Training ProgramT32GM145427 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BRUCE A HAMILTON · 2022 to 2026
$2.6M
Patch-based deep scRNA-Seq to understand axon repair in the mammalian spinal cordR01NS139527 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Binhai Zheng · 2024 to 2026
$1.5M
STELLARIS 8 DIVE Multiphoton MicroscopeS10OD036455 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ZHENG, BINHAI · 2025 to 2025
$750k
Elyra 7 Microscope with Lattice SIM2S10OD030505 · OD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ZHENG, BINHAI · 2022 to 2022
$600k
Applying spatial transcriptomics and mouse molecular genetics to understand the mechanisms of fibrotic scarring for promoting neural repair after spinal cord injuryF31NS147852 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LONDONO, CAMILO · 2025 to 2025
$43k
NIGMS NIH HHS T32 GM008666NIGMS NIH HHS T32 GM145427NIH HHS S10 OD030505NIH HHS S10 OD036455NINDS NIH HHS F31 NS147852NINDS NIH HHS P30 NS047101NINDS NIH HHS R01 NS093055NINDS NIH HHS R01 NS139527
6 · The paper itself

Abstract

Quantitative analysis of corticospinal tract (CST) sprouting after injury requires reliable labeling of long-range axons and fine collateral branches. Conventional biotinylated dextran amine (BDA) tracing has limited sensitivity and requires additional surgeries, while some viral-based approaches, although robust, rely on extensive tissue processing and signal amplification. Here, we describe a streamlined adeno-associated virus (AAV)-based workflow for CST sprouting analysis using TurboRFP that enables robust labeling of descending CST axons, including sprouting fibers after unilateral pyramidotomy. This approach allows direct visualization of fine CST axons without immunostaining or signal amplification, simplifying tissue processing and reducing experimental variability. Using a standardized workflow, we enable consistent CST labeling and reproducible quantification of CST remodeling. In addition, compatibility with co-delivery of other AAVs enables simultaneous circuit tracing and genetic manipulation within the same neuronal population. This workflow provides a practical platform that lowers technical barriers to axon repair research and potentially improves reproducibility across laboratories.

Indexed as

DependovirusPyramidal TractsAnimalsAxonsGenetic VectorsHumansMiceNerve RegenerationAAV vectorsaxon sproutingaxon tracingCNS injurycorticospinal tractCP: imagingCP: neuroscienceCSTfluorescent labelingneural repairpyramidotomy

Identifiers

PMID42537650
PMCPMC13615489

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.