ArticlePhotochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology2026
Determining molar absorption coefficients for chromoproteins using relative actinometry with transient absorption spectroscopy: application to Orange Carotenoid Protein.
Article in Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Determining molar absorption coefficient for chromoprotein samples containing a significant fraction of apoprotein is problematic since conventional protein quantification methods, such as the Bradford assay, determine the total amount of protein. This leads to underestimation of molar absorption coefficient. Using Orange Carotenoid Protein (OCP) as a model system, we demonstrate an alternative approach employing the relative actinometry method and femtosecond transient absorption spectroscopy. By comparing the ground state bleaching bands of OCP and β-carotene recorded immediately after the excitation, before the onset of decay, we determined a molar absorption coefficient that is representative of the holoprotein. β-carotene serves as a well-characterized reference compound with photophysics similar to that of OCP, enabling error cancellation. We obtained a value of 110,000 M
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