Evidence map›Paper›PMID 42536213›Full record

ArticleMolecular biology reports2026

Reference gene selection for gene expression analysis in Coffea arabica L. under ABA and gibberellin treatments.

Lillian Magalhães Azevedo, Robert Márquez-Gutiérrez, Matheus Martins Daúde, Horllys Gomes Barreto, Renato Ribeiro de Lima, Raphael Ricon de Oliveira, Antonio Chalfun-Junior

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Lillian Magalhães AzevedoCentral Laboratory of Molecular Biology (LCBM), Institute of Natural Science (ICN), Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil.
Robert Márquez-GutiérrezLaboratory of Plant Molecular Physiology (LFMP), Plant Physiology Sector, Institute of Natural Science (ICN), Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil.
Matheus Martins DaúdeLaboratory of Molecular Analysis (LAM), Life Sciences Department, Federal University of Tocantins, Palmas, Tocantins, Brazil.
Horllys Gomes BarretoLaboratory of Molecular Analysis (LAM), Life Sciences Department, Federal University of Tocantins, Palmas, Tocantins, Brazil.
Renato Ribeiro de LimaStatistics Department, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil.
Raphael Ricon de OliveiraLaboratory of Plant Molecular Physiology (LFMP), Plant Physiology Sector, Institute of Natural Science (ICN), Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil.
Antonio Chalfun-JuniorLaboratory of Plant Molecular Physiology (LFMP), Plant Physiology Sector, Institute of Natural Science (ICN), Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil. chalfunjunior@ufla.br.ORCID https://orcid.org/0000-0002-1794-9429

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundQuantitative reverse transcription polymerase chain reaction (RT-qPCR) requires stable reference genes (RGs) to ensure accurate target gene normalization. Plant growth regulators like abscisic acid (ABA) and gibberellins (GA METHODS AND

resultsThe stability of CaACT, CaGAPDH, CaEFA1, CaTUB, CaAP47, CaMDH, CaRPL39, and CaUBQ2 was evaluated in leaf tissues of Coffea arabica during the reproductive stage under different ABA and GA

conclusionsCaACT and CaMDH are the most suitable RGs for RT-qPCR normalization in leaf tissues of Coffea arabica during the reproductive stage under ABA and GA

Indexed as

Abscisic AcidCoffeaGene Expression ProfilingGibberellinsGene Expression Regulation, PlantGenes, PlantPlant Growth RegulatorsPlant LeavesPlant ProteinsReal-Time Polymerase Chain ReactionReference StandardsAbscisic AcidGibberellinsPlant Growth RegulatorsPlant ProteinsCoffee plantsGA3 and ABA treatmentsGene expressionReference genes stability

Identifiers

PMID42536213
PMCPMC13427861

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.