Dual-readout lateral flow immunoassay based on upconversion nanoparticle-gold nanoparticle luminescence resonance energy transfer for rapid determination of 17β-estradiol.

Yechen Cheng, Chaofan Zhang, Xinfeng Dai, Xinrui Yuan, Jiao Cao, Bo Tian, Jinliang Liu

Abstract read

In one paragraph

Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Yechen Cheng *School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, P. R. China.

Chaofan Zhang *School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, P. R. China.

Xinfeng DaiSchool of Life Sciences, Shanghai University, Shanghai, 200444, P. R. China.

Xinrui YuanSchool of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, P. R. China.

Jiao CaoSchool of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, P. R. China.

Bo TianSchool of Life Sciences, Shanghai University, Shanghai, 200444, P. R. China.

Jinliang LiuSchool of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, P. R. China. liujl@shu.edu.cn.ORCID https://orcid.org/0000-0003-1688-5969

Funding

the Natural Science Foundation of Shanghai 21ZR1423200

6 · The paper itself

Abstract

A luminescence resonance energy transfer (LRET)-regulated dual-readout lateral flow immunoassay (LFIA) is reported that integrates naked-eye colorimetric screening and near-infrared (NIR)-excited quantitative luminescence analysis on a single test strip. Core-shell upconversion nanoparticles (UCNPs) functionalized with E2-bovine serum albumin conjugates were immobilized on the test line as luminescent donors, while gold nanoparticle (AuNP)-labeled anti-E2 monoclonal antibodies served dually as colorimetric labels and efficient LRET acceptors. Time-resolved luminescence measurements confirmed an LRET efficiency of 18.8%, with the donor lifetime decreasing from 720 to 585 µs upon immunocomplex formation. Under optimized conditions, the assay was completed within 10 min. The luminescence mode exhibited a linear response over 1-500 ng/mL with a limit of detection of 1.37 ng/mL, while the visual mode enabled naked-eye discrimination at 20-40 ng/mL. This UCNP-AuNP LRET strategy provides a practical dual-readout format that balances operational simplicity and quantitative performance, offering a versatile platform for rapid on-site screening of small-molecule contaminants in complex matrices.

Indexed as

EstradiolGoldMetal NanoparticlesAnimalsAntibodies, ImmobilizedAntibodies, MonoclonalFluorescence Resonance Energy TransferImmunoassayLimit of DetectionLuminescent MeasurementsSerum Albumin, BovineAntibodies, ImmobilizedAntibodies, MonoclonalEstradiolGoldSerum Albumin, Bovine17β-estradiolGold nanoparticlesLateral flow immunoassayLuminescence resonance energy transferUpconversion nanoparticles

Identifiers

PMID42536187

DOI10.1007/s00604-026-08327-6

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.