ArticleFrontiers in microbiology2026
Nanopore sequencing in suitcase lab enables improved detection of
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: The application of next-generation sequencing (NGS) is rapidly expanding for antimicrobial resistance (AMR) surveillance and clinical decision-making. However, despite a high AMR burden, many low- and middle-income countries lack the infrastructure and technical capacity required to implement sequencing-based approaches. To address this gap and demonstrate the practical application of the technology, we compared the performance of nanopore sequencing combined with real-time analysis using the EPI2ME platform in a mobile laboratory with that of a hybrid sequencing approach integrating Illumina short reads and nanopore long reads. Methods: In this exploratory diagnostic evaluation study, 25 Results: A total of 36 Discussion: These findings support the feasibility of deploying nanopore sequencing in mobile suitcase laboratories to strengthen AMR surveillance in food production systems. Given its portability, rapid turnaround time, and minimal infrastructure requirements, the approach may also have broader applications for One Health based AMR surveillance, clinical decision-making, and outbreak investigations in resource-limited settings.
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