Evidence map›Paper›PMID 42533817›Full record

ArticleLab on a chip2026

A biomimetic microphysiological system predicts the impact of sepsis therapeutics on neutrophil-endothelial dynamics.

Dan Liu, Nazgol Esmalian Afyouni, Qingliang Yang, Svetllana Kallogjerovic, Bojana Gligorijevic, Satya Kunapuli, Laurie E Kilpatrick, Mohammad F Kiani

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lab on a chip, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Dan LiuDepartment of Bioengineering, Temple University, Philadelphia, PA 19122, USA. mohammad.kiani@temple.edu.ORCID 0009-0008-1742-4746
Nazgol Esmalian AfyouniDepartment of Mechanical Engineering, Temple University, Philadelphia, PA 19122, USA.ORCID 0009-0003-9325-5650
Qingliang YangDepartment of Mechanical Engineering, Temple University, Philadelphia, PA 19122, USA.
Svetllana KallogjerovicDepartment of Bioengineering, Temple University, Philadelphia, PA 19122, USA. mohammad.kiani@temple.edu.ORCID 0009-0008-0209-8554
Bojana GligorijevicDepartment of Bioengineering, Temple University, Philadelphia, PA 19122, USA. mohammad.kiani@temple.edu.ORCID 0000-0001-9071-7467
Satya KunapuliSol Sherry Thrombosis Research Center, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, USA.
Laurie E KilpatrickCenter for Inflammation and Lung Research, Department of Microbiology, Immunology and Inflammation, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, USA.ORCID 0000-0002-2554-507X
Mohammad F KianiDepartment of Bioengineering, Temple University, Philadelphia, PA 19122, USA. mohammad.kiani@temple.edu.

Funding

Targeting invadopodia-related mechanisms of cancer cell invasion and metastasisR01CA230777 · NCI · TEMPLE UNIV OF THE COMMONWEALTH · PI GLIGORIJEVIC, BOJANA · 2019 to 2023
$1.9M
Functional Immune Phenotyping of Sepsis Patients: Integrating Microphysiological Assays, Omics and In Silico ModelingR01HL181042 · NHLBI · TEMPLE UNIV OF THE COMMONWEALTH · PI MOHAMMAD F KIANI, Laurie E Kilpatrick · 2025 to 2026
$1.3M
A multiorgan microphysiological platform to discover and screen therapeutics for treating radiation induced vascular injuryU01AI195488 · NIAID · TEMPLE UNIV OF THE COMMONWEALTH · PI MOHAMMAD F KIANI, Laurie E Kilpatrick · 2026 to 2026
$555k
NCI NIH HHS R01 CA230777NHLBI NIH HHS R01 HL181042NIAID NIH HHS U01 AI195488
6 · The paper itself

Abstract

Current preclinical models fail to capture human neutrophil-endothelial interactions central to sepsis, contributing to repeated failure of candidate therapeutics in clinical trials. Here, we present a biomimetic microphysiological system (bMPS) integrating primary human endothelial cells, human neutrophils and controlled chemoattractant gradients under physiological flow in a microvascular network. This platform enables real-time visualization of neutrophil adhesion and transmigration, along with quantitative analysis of endothelial barrier integrity. We demonstrate chemoattractant-dependent differential neutrophil recruitment to host-derived IL-8 and bacterial-derived fMLP and differential responses to two different therapeutics: the PAF receptor antagonist BN-52021 reduces recruitment in response to IL-8 but not to fMLP, whereas the PKC-

Indexed as

BiomimeticsEndothelial CellsNeutrophilsSepsisCell AdhesionCells, CulturedHumansInterleukin-8Microphysiological SystemsInterleukin-8

Identifiers

PMID42533817
PMCPMC13425194

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.